Taniguchi Ryo, Shi Lei, Fujii Masae, Ueda Katsura, Honma Shiho, Wakisaka Satoshi
Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, Suita, Japan.
Arch Histol Cytol. 2005 Dec;68(4):243-50. doi: 10.1679/aohc.68.243.
Lectin histochemistry of Jacalin (Artocarpus integrifolia) and peanut agglutinin (PNA), specific lectins for galactosyl (beta-1, 3) N-acetylgalactosamine (galactosyl (beta-1, 3) GalNAc), was applied to the gustatory epithelium of the adult rat. In the ordinary lingual epithelium, Jacalin and PNA labeled the cell membrane from the basal to granular cell layer. They also bound membranes of rounded-cells at the basal portion of taste buds, but the number of PNA labeled cells was smaller than that of Jacalin labeled cells. There was no apparent difference in the binding patterns of Jacalin and PNA among the taste buds of the lingual papillae and those of the palatal epithelium. Occasionally, a few spindle-shaped cells were labeled with Jacalin, but not with PNA. Double labeling of Jacalin and alpha-gustducin, a specific marker for type II cells, revealed that Jacalin-labeled spindle-shaped taste cells were immunonegative for alpha-gustducin. Spindle-shaped cells expressing protein gene product 9.5 (PGP 9.5) immunoreactivity lacked Jacalin labeling. During the development of taste buds in circumvallate papillae, the binding pattern of Jacalin became almost identical from postnatal day 5. The present results indicate that rounded cells at the basal portion of the taste buds cells (type IV cells) bind to Jacalin and PNA, and these lectins are specific markers for type IV cells of the rat taste cells.
将嘉兰凝集素（菠萝蜜）和花生凝集素（PNA）（半乳糖基（β-1,3）N-乙酰半乳糖胺（半乳糖基（β-1,3）GalNAc）的特异性凝集素）的凝集素组织化学方法应用于成年大鼠的味觉上皮。在普通舌上皮中，嘉兰凝集素和花生凝集素标记了从基底细胞层到颗粒细胞层的细胞膜。它们还与味蕾基部圆形细胞的膜结合，但花生凝集素标记的细胞数量少于嘉兰凝集素标记的细胞。舌乳头味蕾和腭上皮味蕾中，嘉兰凝集素和花生凝集素的结合模式没有明显差异。偶尔，一些梭形细胞被嘉兰凝集素标记，但未被花生凝集素标记。嘉兰凝集素与α-味导素（II型细胞的特异性标记物）的双重标记显示，被嘉兰凝集素标记的梭形味觉细胞对α-味导素免疫阴性。表达蛋白基因产物9.5（PGP 9.5）免疫反应性的梭形细胞缺乏嘉兰凝集素标记。在轮廓乳头味蕾发育过程中，从出生后第5天起，嘉兰凝集素的结合模式几乎相同。目前的结果表明，味蕾细胞基部的圆形细胞（IV型细胞）与嘉兰凝集素和花生凝集素结合，这些凝集素是大鼠味觉细胞IV型细胞的特异性标记物。
