Mohanty Sagarika, Dafale Nishant, Rao Nageswara Neti
National Environmental Engineering Research Institute, Nehru Marg, 440020, Nagpur, India.
Biodegradation. 2006 Oct;17(5):403-13. doi: 10.1007/s10532-005-9011-0. Epub 2006 Feb 14.
A two-stage anaerobic-aerobic treatment process based on mixed culture of bacteria isolated from textile dye effluent was used to degrade reactive black 5 dye (RB-5). The anaerobic step was studied in more detail by varying the dye concentration from 100 to 3000 mg l(-1). The results showed that major decolorization was achieved during the anaerobic process. The time required for decolorization by > 90% increased as the concentration of the dye increased. It was also found that maintaining dissolved oxygen (DO) concentration below 0.5 mg l(-1 )and addition of a co-substrate viz., glucose, facilitates anaerobic decolorization reaction remarkably. An attempt was made to identify the metabolites formed in anaerobic process by using high performance liquid chromatography (HPLC) and UV-VIS spectrophotometry. A plate assay was performed for the detection of dominant decolorizing bacteria. Only a few bacterial colonies with high clearing zones (decolorization zones) were found. The results showed that under anaerobic condition RB-5 molecules were reduced and aromatic amines were generated. The aromatic amine metabolite was partly removed in subsequent aerobic bio-treatment. It was possible to achieve more than 90% decolorization and approximately 46% reduction in amine metabolite concentration through two-stage anaerobic-aerobic treatment after a reaction period of 2 days.
采用基于从纺织印染废水中分离出的混合细菌培养物的两级厌氧-好氧处理工艺来降解活性黑5染料（RB-5）。通过将染料浓度从100毫克/升变化到3000毫克/升，对厌氧步骤进行了更详细的研究。结果表明，主要的脱色过程发生在厌氧阶段。脱色率>90%所需的时间随着染料浓度的增加而增加。还发现，将溶解氧（DO）浓度维持在0.5毫克/升以下并添加共底物（即葡萄糖），可显著促进厌氧脱色反应。尝试通过高效液相色谱（HPLC）和紫外-可见分光光度法来鉴定厌氧过程中形成的代谢产物。进行了平板试验以检测主要的脱色细菌。仅发现了少数具有高透明圈（脱色圈）的细菌菌落。结果表明，在厌氧条件下，RB-5分子被还原并生成了芳香胺。芳香胺代谢产物在随后的好氧生物处理中被部分去除。经过2天的反应期，通过两级厌氧-好氧处理，脱色率可以达到90%以上，胺代谢产物浓度可降低约46%。
