Narbutt Joanna, Lesiak Aleksandra, Sysa-Jedrzejowska Anna, Zalewska Anna, Lewy-Trenda Iwona
Department of Dermatology, Medical University, Lódź.
Pol J Pathol. 2005;56(4):155-60.
The evaluation of contact hypersensitivity (CHS) reaction is one of the methods used in the assessment of the immune status of an organism after UV radiation. The aim of the study was to compare usefulness of visual scoring system and histological morphometry in the assessment of CHS response after exposure of humans to solar simulated radiation (SSR). The study included 140 healthy volunteers, 33 people were irradiated for 2 days, 34 - for 10 days and 33 - for 30 days with SSR. Forty non-irradiated individuals served as controls. All the volunteers were sensitized with diphenylocyclopropenone (DPCP) 24 h after final exposure. Statistical analysis comparing intensity of CHS reaction based on visual score between irradiated groups and non-irradiated group revealed no differences (p>0.05). We found a significant difference in epidermal thickness between healthy skin and irradiated groups (p<0.05) and a positive correlation between intensity of spongiosis and clinical score for CHS response at 3.2 DPCP site (p<0.000001). A negative correlation between time of irradiation and spongiosis score was revealed (R=-0.28; p<0.001). We conclude that histological examination of biopsies taken from one of the series of elicitation sites is a reliable and sensitive method in the evaluation of CHS response after UVR.
接触性超敏反应（CHS）的评估是用于评估紫外线辐射后机体免疫状态的方法之一。本研究的目的是比较视觉评分系统和组织学形态计量学在评估人类暴露于太阳模拟辐射（SSR）后的CHS反应中的有用性。该研究纳入了140名健康志愿者，其中33人接受了2天的SSR照射，34人接受了10天的照射，33人接受了30天的照射。40名未照射的个体作为对照。所有志愿者在最后一次暴露24小时后用二苯基环丙烯酮（DPCP）进行致敏。基于视觉评分对照射组和未照射组之间的CHS反应强度进行统计分析，结果显示无差异（p>0.05）。我们发现健康皮肤与照射组之间的表皮厚度存在显著差异（p<0.05），并且在3.2 DPCP部位海绵形成强度与CHS反应临床评分之间存在正相关（p<0.000001）。还发现照射时间与海绵形成评分之间存在负相关（R=-0.28；p<0.001）。我们得出结论，对取自一系列激发部位之一的活检组织进行组织学检查是评估紫外线辐射后CHS反应的一种可靠且敏感的方法。
