McBee Todd W, Wang Liying, Ge Chenhao, Beam Brooke M, Moore Ana L, Gust Devens, Moore Thomas A, Armstrong Neal R, Saavedra S Scott
Department of Chemistry, University of Arizona, Tucson, AZ 85721-0041, USA.
J Am Chem Soc. 2006 Feb 22;128(7):2184-5. doi: 10.1021/ja056750w.
Cellular energy transduction processes are often driven by transmembrane ion gradients, and numerous artificial biomembrane systems have been developed that allow for chemically or light-induced charge transport into/out of liposomes. Liposomal architectures, however, are not readily interfaced to a solid-state transducer. Formation of an ion gradient across a planar-supported membrane, "wired" to a substrate electrode, may ultimately allow utilization of the potential energy to drive other electrochemical processes. Described here is a novel conductive polymer/planar waveguide assembly that provides for highly sensitive transduction of proton transport across a planar-supported lipid bilayer (PSLB). A quinone proton shuttle is embedded in the PSLB, which is coupled to the planar optical waveguide electrode through a pH-sensitive, self-assembled conductive polymer film. Interfacial potential and absorbance changes in the conductive polymer film provide for sensitive characterization of transmembrane proton transport. The general and flexible nature of this architecture makes it adaptable to many different types of transmembrane transport chemistries, particularly light-activated systems.
细胞能量转导过程通常由跨膜离子梯度驱动，并且已经开发出许多人工生物膜系统，这些系统允许化学或光诱导的电荷进出脂质体。然而，脂质体结构不易与固态换能器连接。在与基底电极“连接”的平面支撑膜上形成离子梯度，最终可能允许利用势能来驱动其他电化学过程。本文描述了一种新型导电聚合物/平面波导组件，它能够对质子跨平面支撑脂质双层（PSLB）的传输进行高度灵敏的转换。醌质子穿梭体嵌入在PSLB中，通过对pH敏感的自组装导电聚合物膜与平面光波导电极耦合。导电聚合物膜中的界面电位和吸光度变化为跨膜质子传输提供了灵敏的表征。这种结构的通用性和灵活性使其适用于许多不同类型的跨膜传输化学过程，特别是光激活系统。
