Swann A C
Department of Psychiatry, University of Texas Medical School, Houston 77225.
Brain Res. 1991 Mar 15;543(2):251-5. doi: 10.1016/0006-8993(91)90035-t.
We investigated the effects of intracerebroventricular infusion of tetrodotoxin on activity and function of brain Na+,K(+)-ATPase. Infusion of 1 or 3 micrograms/h for 2, 4 or 7 days by osmotic minipump reduced the number of Na+,K(+)-ATPase sites as measured by ouabain binding in cerebral cortex. Tetrodotoxin infusions substantially reduced the functional transport capacity of Na+,K(+)-ATPase, measured by the maximal increase in synaptoneurosomal 86Rb+ uptake in the presence of monensin. The effects were maximal at 4 days, with a possible partial recovery of activity at 7 days. Results of ouabain inhibition curves suggested that the effect of tetrodotoxin was not specific for enzyme with high or low affinity for ouabain.
我们研究了脑室内注入河豚毒素对脑Na⁺,K⁺-ATP酶活性和功能的影响。通过渗透微型泵以1或3微克/小时的速度注入2、4或7天，可减少通过哇巴因结合法测定的大脑皮质中Na⁺,K⁺-ATP酶位点的数量。注入河豚毒素可显著降低Na⁺,K⁺-ATP酶的功能转运能力，这是通过在莫能菌素存在下突触神经体中⁸⁶Rb⁺摄取的最大增加来衡量的。效应在4天时最大，7天时活性可能部分恢复。哇巴因抑制曲线结果表明，河豚毒素的作用并非对与哇巴因具有高亲和力或低亲和力的酶具有特异性。
