线粒体DNA拷贝数与肝细胞癌临床病理参数的相关性
Correlation between copy number of mitochondrial DNA and clinico-pathologic parameters of hepatocellular carcinoma.
作者信息
Yamada S, Nomoto S, Fujii T, Kaneko T, Takeda S, Inoue S, Kanazumi N, Nakao A
机构信息
Department of Surgery II, Graduate School and Faculty of Medicine, University of Nagoya, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan.
出版信息
Eur J Surg Oncol. 2006 Apr;32(3):303-7. doi: 10.1016/j.ejso.2006.01.002. Epub 2006 Feb 14.
AIMS
In the current study, we investigated possible correlations of the mtDNA copy number in hepatocellular carcinoma (HCC) with the pathological findings and prognosis.
METHODS
We studied 31 HCC specimens using quantitative real-time polymerase chain reaction analysis, and the correlation between the mtDNA copy number and the clinicopathologic parameters and mutations in the D-loop region of the mitochondrial genome.
RESULTS
The mtDNA copy number was reduced in HCCs compared with the corresponding non-cancerous liver tissues (p=0.002), and significantly correlated with tumour size (p=0.014) and cirrhosis (p=0.048). Patients with a low mtDNA copy number tended to show shorter 5-year survival rates than patients with a high mtDNA copy number when assessed by Kaplan-Meier curves, but not a significant (overall survival rate, 63 vs 83%; p=0.19). The copy number of HCC with mtDNA D-loop mutation or deletion was lower, but not significantly so (p=0.656, p=0.590, respectively).
CONCLUSIONS
Our results indicated that a reduced copy number of mtDNA is correlated with HCC and associated with malignant potential.
目的
在本研究中，我们调查了肝细胞癌（HCC）中线粒体DNA（mtDNA）拷贝数与病理结果及预后之间可能存在的相关性。
方法
我们使用定量实时聚合酶链反应分析研究了31例HCC标本，并分析了mtDNA拷贝数与临床病理参数以及线粒体基因组D环区域突变之间的相关性。
结果
与相应的癌旁肝组织相比，HCC中的mtDNA拷贝数降低（p = 0.002），并且与肿瘤大小（p = 0.014）和肝硬化（p = 该文档包含了肝细胞癌中mtDNA拷贝数与病理结果和预后的相关性研究，结果显示HCC中mtDNA拷贝数降低，与肿瘤大小和肝硬化相关，低mtDNA拷贝数患者5年生存率较低但无统计学意义，mtDNA D环突变或缺失的HCC拷贝数较低也无统计学意义。048）显著相关。通过Kaplan-Meier曲线评估时，mtDNA拷贝数低的患者5年生存率往往比mtDNA拷贝数高的患者短，但差异无统计学意义（总生存率分别为63%和83%；p = 0.19）。mtDNA D环突变或缺失的HCC拷贝数较低，但差异无统计学意义（分别为p = 0.656，p = 0.590）。
结论
我们的结果表明，mtDNA拷贝数减少与HCC相关，并与恶性潜能有关。
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