通过二维电泳-质谱联用技术对康氏立克次体和普氏立克次体进行蛋白质组分析：迈向立克次体基因组功能分析的一步。

Rickettsia conorii and R. prowazekii proteome analysis by 2DE-MS: a step toward functional analysis of rickettsial genomes.

作者信息

Renesto Patricia, Azza Saïd, Dolla Alain, Fourquet Patrick, Vestris Guy, Gorvel Jean-Pierre, Raoult Didier

机构信息

Unité des Rickettsies, CNRS UMR 6020, Marseille, France.

出版信息

Ann N Y Acad Sci. 2005 Dec;1063:90-3. doi: 10.1196/annals.1355.014.

DOI:10.1196/annals.1355.014

PMID:16481497

Abstract

In this work, we present a comparative two-dimensional (2D) PAGE analysis of Rickettsia conorii and Rickettsia prowazekii. This analysis reveals protein spots that were either unique to or common to both strains, some of them being identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry.

摘要

在这项工作中，我们展示了康氏立克次体和普氏立克次体的二维（2D）聚丙烯酰胺凝胶电泳（PAGE）对比分析。该分析揭示了两种菌株特有的或共有的蛋白质斑点，其中一些通过基质辅助激光解吸电离飞行时间质谱法得以鉴定。