剖析普氏立克次氏体基因组：遗传与蛋白质组学方法

Dissecting the Rickettsia prowazekii genome: genetic and proteomic approaches.

作者信息

Tucker Aimee M, Pannell Lewis K, Wood David O

机构信息

Department of Microbiology and Immunology, Laboratory of Molecular Biology, University of South Alabama, Mobile, AL 36688, USA.

出版信息

Ann N Y Acad Sci. 2005 Dec;1063:35-46. doi: 10.1196/annals.1355.005.

DOI:10.1196/annals.1355.005

PMID:16481488

Abstract

The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high-throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.

摘要

普氏立克次体在细胞内生长的专性对分析立克次体基因功能和基因表达造成了严重限制。幸运的是，随着针对这种迷人的人类病原体的基因操作和蛋白质组分析方法的出现，这种情况正在改善。在本文中，我们综述了立克次体遗传学的现状以及使用遗传方法分离立克次体突变体的情况。此外，还将描述通过立克次体蛋白质组特征来检测立克次体基因表达的情况。这将包括对一种高通量、精确质量方法的描述，该方法已在复杂的立克次体蛋白质样本中鉴定出596种立克次体蛋白质。