Ge Hong, Tong Min, Li Andrew, Mehta Rajan, Ching Wei-Mei
Rickettsial Diseases Department, Naval Medical Research Center, Silver Spring, Maryland 20910, USA.
Ann N Y Acad Sci. 2005 Dec;1063:231-8. doi: 10.1196/annals.1355.036.
The 22-kDa protein antigen is one of several antigens recognized by sera from scrub typhus patients infected with Orientia tsutsugamushi. The 22-kDa protein genes from six O. tsutsugamushi strains (Kato, TA763, AFSC 7, 18-032460, TH1814, MAK119) were cloned and their sequences were determined and compared to each other and to the Karp strain sequence listed in GenBank. The sequence alignment revealed that the promoter regions of these seven strains were highly conserved. However, the ORFs exhibited some variation. The phylogenetic analysis of the DNA sequences indicated that among the seven strains assessed, Kato and TA763 were the most closely related, while Karp and TH1814 were the most distantly related. The information gained from this analysis will facilitate our selection of O. tsutsugamushi strains from which antigens should be derived to be included in a multivalent vaccine candidate for scrub typhus.
22 kDa蛋白抗原是被恙虫病东方体感染的恙虫病患者血清所识别的几种抗原之一。克隆了来自6株恙虫病东方体菌株（Kato、TA763、AFSC 7、18 - 032460、TH1814、MAK119）的22 kDa蛋白基因，测定了它们的序列，并相互比较，同时与GenBank中列出的Karp菌株序列进行比较。序列比对显示这7个菌株的启动子区域高度保守。然而，开放阅读框表现出一些变异。DNA序列的系统发育分析表明，在所评估的7个菌株中，Kato和TA763关系最为密切，而Karp和TH1814关系最为疏远。从该分析中获得的信息将有助于我们选择恙虫病东方体菌株，从中获取抗原，以纳入恙虫病多价候选疫苗。
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