Herradón P G, Razquin B, Zapata A G
Departamento de Patología Animal y Producción Animal, Facultad de Veterinaria, Universidad de Santiago de Compostela, Lugo, Spain.
Am J Anat. 1991 May;191(1):57-66. doi: 10.1002/aja.1001910106.
For the present study we used the classic model of early partial decapitation (DCx) of chicken embryos (Fugo, J. Exp. Zool., 85: 271-298, 1940; Betz, Gen. Comp. Endocrinol., 9: 172-186, 1967) in an attempt to analyze the neuroendocrine immune relationships during ontogeny. The elimination of the prosencephalon in chickens at 33-38 hr of incubation induced profound structural, histochemical, and morphometric changes in the embryonic development of the thymus gland. These included a greater development of the cortex than of the medulla, an increased mitotic index, high numbers of pyknotic cells, and enlarged connective tissue trabeculae containing numerous large lymphoid cells; hypertrophied reticular-epithelial cells; delayed appearance of medullary epithelial cysts; and intrathymic granulopoiesis. Furthermore, preliminary radioimmunoassays revealed a sharp increase in the values of circulating thymic hormones, mainly thymosin beta 4 in 17-day-old embryos. The results are discussed with regard to the possible role of prolactin, thyroxine, testosterone, and thymic hormones in the ontogenic development of the chicken thymus.
在本研究中，我们采用鸡胚早期部分断头（DCx）的经典模型（Fugo，《实验动物学杂志》，85：271 - 298，1940年；Betz，《普通比较内分泌学》，9：172 - 186，1967年），试图分析个体发育过程中的神经内分泌免疫关系。在孵化33 - 38小时的鸡中去除前脑，会在胸腺的胚胎发育过程中引起深刻的结构、组织化学和形态计量学变化。这些变化包括皮质比髓质发育更充分、有丝分裂指数增加、大量固缩细胞、含有大量大淋巴细胞的结缔组织小梁增粗；网状上皮细胞肥大；髓质上皮囊肿出现延迟；以及胸腺内粒细胞生成。此外，初步放射免疫分析显示，在17日龄胚胎中，循环胸腺激素的值急剧增加，主要是胸腺素β4。针对催乳素、甲状腺素、睾酮和胸腺激素在鸡胸腺个体发育中的可能作用对结果进行了讨论。
