Zhai W R
Department of Pathology, Shanghai Medical University.
Zhonghua Bing Li Xue Za Zhi. 1991 Mar;20(1):21-3.
Localization of c-myc gene protein was investigated in human hepatoblastoma cells (Hep G2) and its derived tumor tissue in nude mice by using immunohistochemical method. Revealing by double staining technique, c-myc protein was located in the nuclei of tumor cells at a site different from where the nucleic acid accumulated but disappeared in the mitotic nucleus. It was confirmed to be in the nuclei, next to the chromatin by immunoelectron microscopy. This pattern was not influenced by HBsAg expression in tumor tissue derived from 2.2.15 cells (transfected Hep G2 cell line with HBV genome) and the intensity of c-myc protein became lower in those tumor cells with expression of HBcAg in the nuclei. Significance of the location of c-myc protein is briefly discussed.
采用免疫组织化学方法，对人肝癌细胞（Hep G2）及其在裸鼠体内形成的肿瘤组织中的c-myc基因蛋白进行定位研究。通过双重染色技术显示，c-myc蛋白位于肿瘤细胞核内，其位置与核酸聚集处不同，且在有丝分裂细胞核中消失。免疫电子显微镜证实其位于细胞核内，靠近染色质。这种模式不受源自2.2.15细胞（用乙肝病毒基因组转染的Hep G2细胞系）的肿瘤组织中HBsAg表达的影响，并且在细胞核中表达HBcAg的那些肿瘤细胞中，c-myc蛋白的强度降低。简要讨论了c-myc蛋白定位的意义。
