Silveira Silvana T, Oliveira Melissa S, Costa Jorge A V, Kalil Susana J
Department of Chemistry, Federal University Foundation of Rio Grande, 96201-900, Rio Grande, RS, Brazil.
Appl Biochem Biotechnol. 2006 Feb;128(2):131-40. doi: 10.1385/abab:128:2:131.
Glucoamylase production by Aspergillus niger in solid-state fermentation was optimized using factorial design and response surface techniques. The variables evaluated were pH and bed thickness in tray, having as response enzyme production and productivity. The bed thickness in tray was the most significant variable for both responses. The highest values for glucoamylase production occurred using pH 4.5 and bed thickness in the inferior limits at 2.0-4.2 cm. For productivity, the optimal conditions were at pH 4.5 as well and bed thickness from 4.4 to 7.5 cm. The optimal conditions for glucoamylase production while obtaining high activity without loss of productivity were pH 4.5 and bed thickness in tray from 4.0 to 4.5 cm, which resulted in an enzyme production of 695 U/g and productivity of 5791 U/h.
采用析因设计和响应面技术优化了黑曲霉在固态发酵中生产糖化酶的工艺。所评估的变量为pH值和托盘床层厚度，响应指标为酶产量和生产率。托盘床层厚度对两个响应指标来说都是最显著的变量。糖化酶产量最高时的条件是pH值为4.5，床层厚度处于下限2.0 - 4.2厘米。对于生产率而言，最佳条件同样是pH值为4.5，床层厚度为4.4至7.5厘米。在不损失生产率的情况下获得高活性糖化酶的最佳生产条件是pH值为4.5，托盘床层厚度为4.0至4.5厘米，此时酶产量为695 U/g，生产率为5791 U/h。
