Taki Hironori, Syutsubo Kazuaki, Mattison Richard G, Harayama Shigeaki
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan.
Biodegradation. 2007 Feb;18(1):17-26. doi: 10.1007/s10532-005-9030-x. Epub 2006 Feb 17.
Soils contaminated with o-xylene were more difficult to bioremediate than those contaminated with other BTEX hydrocarbons (benzene, toluene, ethylbenzene, m-xylene and p-xylene). In order to identify microorganisms responsible for o-xylene degradation in soil, microbial community structure analyses were carried out with two soil samples in the presence of o-xylene and mineral nutrients. In two different soil samples, Rhodococcus opacus became abundant. We were also able to isolate o-xylene degrading Rhodococcus species from these soil samples. A primer set was developed to specifically detect a cluster of this Rhodococcus group including isolated Rhodococcus strains, Rhodococcus opacus and Rhodococcus koreensis. The growth of this bacterial group in an o-xylene-contaminated soil was followed by competitive PCR (cPCR). The decrease in o-xylene clearly paralleled the growth of the Rhodococcus group.
被邻二甲苯污染的土壤比被其他BTEX碳氢化合物（苯、甲苯、乙苯、间二甲苯和对二甲苯）污染的土壤更难进行生物修复。为了鉴定土壤中负责邻二甲苯降解的微生物，在邻二甲苯和矿物养分存在的情况下，对两个土壤样品进行了微生物群落结构分析。在两个不同的土壤样品中，不透明红球菌变得丰富起来。我们还能够从这些土壤样品中分离出降解邻二甲苯的红球菌物种。开发了一套引物，以特异性检测包括分离出的红球菌菌株、不透明红球菌和韩国红球菌在内的这一红球菌群体。通过竞争性PCR（cPCR）跟踪该细菌群体在被邻二甲苯污染的土壤中的生长情况。邻二甲苯的减少明显与红球菌群体的生长平行。
