结核分枝杆菌吲哚-3-甘油磷酸合酶的纯化与鉴定
Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase.
作者信息
Yang Yanping, Zhang Min, Zhang Hongmei, Lei Jianqiang, Jin Ruiliang, Xu Shengfeng, Bao Jialing, Zhang Lu, Wang Honghai
机构信息
State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, PR China.
出版信息
Biochemistry (Mosc). 2006;71 Suppl 1:S38-43. doi: 10.1134/s0006297906130062.
Indole-3-glycerol phosphate synthase (IGPS) plays an important role in the survival of Mycobacterium tuberculosis. The trpC gene, encoding IGPS, is essential for the growth of M. tuberculosis. It was expressed at the transcriptional level in cultured M. tuberculosis. The recombinant IGPS with an added His-tag was purified. The His-tag was found to have a small effect on the biochemical properties of IGPS. IGPS is a monofunctional enzyme in M. tuberculosis. Recombinant IGPS has considerable beta-pleated sheet and is relatively compact. The enzyme activity is significantly inhibited by denaturants and antibiotics, suggesting that IGPS may be a novel potential drug target of M. tuberculosis.
吲哚 - 3 - 磷酸甘油合酶（IGPS）在结核分枝杆菌的存活中起重要作用。编码IGPS的trpC基因对结核分枝杆菌的生长至关重要。它在培养的结核分枝杆菌中在转录水平表达。添加了His标签的重组IGPS被纯化。发现His标签对IGPS的生化特性影响较小。IGPS在结核分枝杆菌中是一种单功能酶。重组IGPS具有相当多的β - 折叠片层且相对紧凑。该酶活性受到变性剂和抗生素的显著抑制，这表明IGPS可能是结核分枝杆菌的一个新的潜在药物靶点。
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