Saville Stephen P, Thomas Derek P, López Ribot José L
Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA.
FEMS Microbiol Lett. 2006 Mar;256(1):151-8. doi: 10.1111/j.1574-6968.2006.00109.x.
Candida albicans interactions with extracellular matrices extracellular matrices represent hallmarks of invasive candidiasis. We have assessed the role of Efg1p, a key transcriptional regulator, in the interactions between C. albicans and extracellular matrices. Wild-type C. albicans cells were able to bind and subsequently penetrate the extracellular matrices layer, whereas the Deltaefg1/Deltaefg1 mutant strain showed a drastically reduced ability to interact with extracellular matrices. Results indicate that, besides its control of morphogenesis, Efg1p also regulates C. albicans interactions with extracellular matrices. Proteomic analyses indicated that expression of 30 surface-associated proteins is affected in the Deltaefg1/Deltaefg1 mutant, some of which may be involved in adherence to extracellular matrices.
白色念珠菌与细胞外基质的相互作用 细胞外基质是侵袭性念珠菌病的标志。我们评估了关键转录调节因子Efg1p在白色念珠菌与细胞外基质相互作用中的作用。野生型白色念珠菌细胞能够结合并随后穿透细胞外基质层，而Deltaefg1/Deltaefg1突变株与细胞外基质相互作用的能力则大幅降低。结果表明，除了控制形态发生外，Efg1p还调节白色念珠菌与细胞外基质的相互作用。蛋白质组学分析表明，Deltaefg1/Deltaefg1突变体中30种表面相关蛋白的表达受到影响，其中一些可能参与了与细胞外基质的黏附。
