Tanee F S F, Njamen D, Magne Ndé C B, Wanji J, Zierau O, Fomum Z T, Vollmer G
Department of Organic Chemistry, University of Yaoundé I, P.O. Box 812 Yaoundé, Cameroon.
Phytomedicine. 2007 Feb;14(2-3):222-6. doi: 10.1016/j.phymed.2006.01.012. Epub 2006 Feb 17.
The stem bark of Erythrina lysistemon, one of the traditionally used "women remedies", has been assessed for its estrogenic activity. The ethyl-acetate extract of the stem bark of E. lysistemon showed estrogenic activities in vitro either in a yeast-based estrogen receptor assay or on the estrogen-dependent stimulation of alkaline phosphatase activity in the human endometrial carcinoma cell line Ishikawa. The estrogenic activity was investigated in vivo in young ovariectomized Wistar female rats after a 7-day treatment. The estrogenicity was evaluated through the proliferative status of target sex organs such as uterus and vagina. The results obtained showed that oral administration of 200 mg/kg BW/d of E. lysistemon extract in comparison to untreated ovariectomized rats significantly increased the vaginal epithelial height by 47.23% (from 8.71+/-0.47 to 12.34+/-1.31 microm); and induced a weak increase of uterine epithelial height by 6.76% (from 5.42+/-0.52 to 5.84+/-0.91 microm). Both were not as pronounced as those elicited in the positive control of 100 microg/kg BW/d of ethinylestradiol given orally. Overall our results suggest that the extract of E. lysistemon contains secondary metabolites endowed with estrogenic activity.
刺桐（Erythrina lysistemon）是一种传统上用于“女性疗法”的植物，其茎皮已被评估雌激素活性。刺桐茎皮的乙酸乙酯提取物在体外酵母雌激素受体试验或人子宫内膜癌细胞系石川细胞中雌激素依赖性碱性磷酸酶活性刺激试验中均显示出雌激素活性。在7天的治疗后，对年轻的去卵巢Wistar雌性大鼠进行了体内雌激素活性研究。通过子宫和阴道等靶性器官的增殖状态来评估雌激素活性。结果表明，与未处理的去卵巢大鼠相比，口服200mg/kg体重/天的刺桐提取物可使阴道上皮高度显著增加47.23%（从8.71±0.47增加到12.34±1.31微米）；并使子宫上皮高度微弱增加6.76%（从5.42±0.52增加到5.84±0.91微米）。两者均不如口服100μg/kg体重/天乙炔雌二醇的阳性对照明显。总体而言，我们的结果表明，刺桐提取物含有具有雌激素活性的次生代谢产物。
