小鼠鳞状细胞癌对光动力疗法联合低剂量顺铂给药的敏感性增强。
Enhanced susceptibility of mouse squamous cell carcinoma to photodynamic therapy combined with low-dose administration of cisplatin.
作者信息
Uehara Masataka, Inokuchi Tsugio, Ikeda Hisazumi
机构信息
Division of Oral and Maxillofacial Surgical Reconstruction and Restoration, Department of Developmental and Reconstructive Medicine, Graduate School of Biomedical Sciences, Nagasaki University, Sakamoto, Nagasaki, Japan.
出版信息
J Oral Maxillofac Surg. 2006 Mar;64(3):390-6. doi: 10.1016/j.joms.2005.11.011.
PURPOSE
We have investigated the antitumor effect of photodynamic therapy (PDT), using Photofrin as the photosensitizer, combined with low-dose cisplatin (CDDP) on NR-S1 mouse squamous cell carcinoma.
MATERIALS AND METHODS
CDDP (5 mg/kg body weight) was injected intraperitoneally either 1 hour or 3 hours prior to PDT or immediately afterward. Twenty-four hours after each protocol, the antitumor effects were evaluated by percentage area of the tumor necrosis in hematoxylin-eosin stained specimens as well as terminal deoxynucleotidyl transferase-mediated d-UTP nick-end labeling indices. Furthermore, the tumor sizes were evaluated at 3, 7, and 10 days after each protocol.
RESULTS
The antitumor effect of PDT was enhanced by administration of CDDP 3 hours before PDT, whereas the administration of CDDP 1 hour before PDT or immediately after PDT did not potentiate a PDT antitumor effect.
CONCLUSION
Administration of low-dose CDDP 3 hours before PDT appears to be a useful treatment modality.
目的
我们研究了以卟吩姆钠作为光敏剂的光动力疗法（PDT）联合低剂量顺铂（CDDP）对NR-S1小鼠鳞状细胞癌的抗肿瘤作用。
材料与方法
在PDT前1小时或3小时或之后立即腹腔注射CDDP（5毫克/千克体重）。每种方案实施24小时后，通过苏木精-伊红染色标本中肿瘤坏死面积百分比以及末端脱氧核苷酸转移酶介导的d-UTP缺口末端标记指数评估抗肿瘤效果。此外，在每种方案实施后的第3、7和10天评估肿瘤大小。
结果
在PDT前3小时给予CDDP可增强PDT的抗肿瘤作用，而在PDT前1小时或PDT后立即给予CDDP不会增强PDT的抗肿瘤作用。
结论
在PDT前3小时给予低剂量CDDP似乎是一种有效的治疗方式。
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