Pereira Lopes Fátima Rosalina, Camargo de Moura Campos Lenira, Dias Corrêa José, Balduino Alex, Lora Silvano, Langone Francesco, Borojevic Radovan, Blanco Martinez Ana Maria
Departamento de Histologia e Embriologia, Instituto de Ciências Biomédicas, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Brasil.
Exp Neurol. 2006 Apr;198(2):457-68. doi: 10.1016/j.expneurol.2005.12.019. Epub 2006 Feb 20.
We evaluated peripheral nerve regeneration using a tubular nerve guide of resorbable collagen filled with either bone marrow-derived cells (BMDCs) in Dulbecco's cell culture medium (DMEM) or with DMEM alone (control). The control group received just the culture medium (vehicle). The left sciatic nerves of ten isogenic mice were transected and the tubular nerve guides were sutured to the end of the proximal and distal nerve stumps. Motor function was tested at 2, 4 and 6 weeks after surgery using the walking track test. The pawprints were analyzed and the print lengths (PL) were measured to evaluate functional recovery. After 6 weeks, mice were anesthetized, perfused transcardially with fixative containing aldehydes, and the sciatic nerves and tubes were dissected and processed for scanning and transmission electron microscopy. Scanning electron microscopy of the collagen tube revealed that the tube wall became progressively thinner after surgery, proving that the tube can be resorbed in vivo. Quantitative analysis of the regenerating nerves showed that the number of myelinated fibers and the myelin area were significantly increased in the experimental group. Also, motor function recovery was faster in animals that received the cell grafts. These results indicate that the collagen tube filled with BMDCs provided an adequate and favorable environment for the growth and myelination of regenerating axons compared to the collagen tube alone.
我们使用可吸收胶原蛋白制成的管状神经导管评估周围神经再生情况，该导管填充有处于杜尔贝科细胞培养基（DMEM）中的骨髓源细胞（BMDCs）或仅填充有DMEM（对照组）。对照组仅接受培养基（赋形剂）。将十只同基因小鼠的左侧坐骨神经切断，把管状神经导管缝合到近端和远端神经残端的末端。在术后2周、4周和6周使用行走轨迹测试来检测运动功能。分析爪印并测量印长（PL）以评估功能恢复情况。6周后，对小鼠进行麻醉，经心脏灌注含醛类的固定剂，然后解剖坐骨神经和导管并进行扫描电子显微镜和透射电子显微镜检查。对胶原管的扫描电子显微镜检查显示，术后管壁逐渐变薄，证明该导管可在体内被吸收。对再生神经的定量分析表明，实验组中髓鞘化纤维的数量和髓鞘面积显著增加。此外，接受细胞移植的动物运动功能恢复更快。这些结果表明，与单独的胶原管相比，填充有BMDCs的胶原管为再生轴突的生长和髓鞘化提供了适宜且有利的环境。
