Hartmann Anke, Boukamp Petra, Friedl Peter
Department of Dermatology and Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany.
Blood Cells Mol Dis. 2006 Mar-Apr;36(2):191-3. doi: 10.1016/j.bcmd.2005.12.033. Epub 2006 Feb 17.
The reconstruction of extracellular matrix (ECM) by confocal reflection microscopy is a physical approach for monitoring the ECM structure, cell-matrix interactions and changes of ECM structure over time. We here show that confocal reflection imaging is useful in reconstructing the fibrillar architecture of 3D fibrin lattices and fibroblasts embedded therein at high resolution up to 250 nm. Together with confocal fluorescence microscopy of collagen deposited by fibroblasts, this technique allows the monitoring of fibrin remodeling by stromal cells. In conclusion, confocal reflection microscopy is a valuable technique for real-time monitoring of the remodeling of fibrin matrices, such as during thrombus formation, wound healing, and tumor formation.
通过共聚焦反射显微镜对细胞外基质（ECM）进行重建，是一种用于监测ECM结构、细胞-基质相互作用以及ECM结构随时间变化的物理方法。我们在此表明，共聚焦反射成像可用于以高达250 nm的高分辨率重建三维纤维蛋白晶格及其内部嵌入的成纤维细胞的纤维结构。结合对成纤维细胞沉积的胶原蛋白进行共聚焦荧光显微镜观察，该技术能够监测基质细胞对纤维蛋白的重塑过程。总之，共聚焦反射显微镜是一种用于实时监测纤维蛋白基质重塑的有价值技术，例如在血栓形成、伤口愈合和肿瘤形成过程中。
