Sensory mediation of female-male mounting in the rat: II. Role of tactile and conspecific cues.

Although previous research has shown that olfactory cues mediate female-male mounting (FMM) in the rat, the role of other sensory modalities on FMM has not been investigated. The present study examined the display of female mounting of castrated male rats in bilevel chambers following different tactile or locomotor activity manipulations. Female rats (N = 40) were ovariectomized (OVX), primed with estrogen (E) and progesterone (P), and given either vaginocervical stimulation (VCS), flank/perineum stimulation (FPS), combined VCS and FPS, or general handling, immediately before each test with a castrated male rat for five trials. Compared to handling, the FPS females showed an increase in FMM behavior, whereas females given VCS, or combined VCS and FPS, showed a decrease in FMM behavior. A second experiment examined the effect of a 15-min delay between stimulation and testing using identical experimental and control conditions. There were no significant differences in the amount of FMM behavior between these groups. Finally, OVX rats primed with E and P were tested with castrated males that had been given injections of a ketamine/xylazine anesthetic mixture, saline, or amphetamine, to induce three levels of conspecific locomotor activity: none, moderate, or high, respectively. A positive linear relationship was found between male activity level and FMM. These data indicate that both tactile cues and cues associated with locomotor activity of the stimulus male modulate FMM.