Nofrarías M, Pujols J, Segalés J, Gibert X, Majó N
Departament de Sanitat i d'Anatomia Animals, Centre de Recerca en Sanitat Animal, CReSA, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.
Res Vet Sci. 2006 Oct;81(2):211-4. doi: 10.1016/j.rvsc.2005.12.005. Epub 2006 Feb 20.
The objective of the study was to analyze changes in peripheral blood leukocyte subsets in cases of naturally occurring exudative epidermitis (EE) in pigs. Five of ten piglets developed the chronic clinical form of EE 2-5 days after weaning (PW). Blood samples were obtained at 7, 14 and 21 days from both normal and clinically affected piglets for routine haematology and for the determination of CD45, CD21, CD4, CD8 and gammadeltaTCR cell markers by flow cytometry. When compared with clinically normal piglets EE affected pigs showed significantly decreased values of monocytes at 14 and 21 days PW, and increased numbers of neutrophils and leukocytes at 21 days PW. The EE affected pigs also had an early significant CD4(+) and CD8(high+) T lymphocyte proliferative response at 7 days PW. However affected pigs had a significantly reduced number of B (CD21(+)) and gammadeltaTCR(+) T lymphocytes in blood at 21 days PW. Although all values remained within the normal range, the significant differences in some peripheral blood leukocyte subsets between the two groups of piglets suggest that the generalised cutaneous infection with Staphylococcus hyicus is severe enough to induce a systemic inflammatory and immune responses.
本研究的目的是分析自然发生的猪渗出性皮炎（EE）病例外周血白细胞亚群的变化。十头仔猪中有五头在断奶后2 - 5天出现了慢性临床形式的EE。在第7天、14天和21天从正常和临床受影响的仔猪采集血样，用于常规血液学检查以及通过流式细胞术测定CD45、CD21、CD4、CD8和γδTCR细胞标志物。与临床正常仔猪相比，EE感染猪在断奶后14天和21天单核细胞值显著降低，在断奶后21天中性粒细胞和白细胞数量增加。EE感染猪在断奶后7天也有早期显著的CD4(+)和CD8(high+) T淋巴细胞增殖反应。然而，感染猪在断奶后21天血液中B（CD21(+)）和γδTCR(+) T淋巴细胞数量显著减少。尽管所有值仍在正常范围内，但两组仔猪外周血白细胞亚群的显著差异表明，猪葡萄球菌的全身性皮肤感染严重到足以引发全身炎症和免疫反应。
