甲状腺激素对大鼠肝线粒体bc1复合物中抗霉素A阻断旁路的影响。
Effects of thyroid hormones on the bypasses of the antimycin A block in the bc1 complex of rat liver mitochondria.
作者信息
Horrum M A, Tobin R B, Ecklund R E
机构信息
Department of Internal Medicine, College of Medicine, University of Nebraska Medical Center, Omaha.
出版信息
Biochem Biophys Res Commun. 1991 Jul 15;178(1):73-8. doi: 10.1016/0006-291x(91)91781-7.
The effect of thyroid hormones on the electron flow through the bc1 complex of rat liver mitochondria was studied using two dye bypasses of the Antimycin A block of the bc1 complex by the method of Alexandre and Lehninger (Biochim. Biophys. Acta 767:120; 1984). Bypass respiration rates with both DCIP (2,6-dichlorophenolindophenol) and TMPD (N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride) were elevated in the hyperthyroid rats and depressed in the hypothyroid groups compared to the euthyroid controls. T3 treatment of hypothyroid rats returned the bypass rates to control levels in 24 hours with the TMPD dye but not for the DCIP. This further demonstrates that different portions of the bc1 complex respond individually to the thyroid state.
采用亚历山大和莱宁格的方法（《生物化学与生物物理学报》767:120；1984年），利用两种染料绕过抗霉素A对bc1复合物的阻断作用，研究了甲状腺激素对大鼠肝脏线粒体中bc1复合物电子传递的影响。与甲状腺功能正常的对照组相比，甲状腺功能亢进大鼠中使用2,6 - 二氯酚靛酚（DCIP）和N,N,N',N'-四甲基对苯二胺二盐酸盐（TMPD）的旁路呼吸速率均升高，而甲状腺功能减退组则降低。用T3处理甲状腺功能减退大鼠24小时后，TMPD染料的旁路速率恢复到对照水平，但DCIP的未恢复。这进一步证明bc1复合物的不同部分对甲状腺状态有不同的反应。
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