Yasuhara T, Mori M, Wakamatsu K, Kubo K
Tsukuba Research Laboratories, Takeda Chemical Industries, Ltd., Japan.
Biochem Biophys Res Commun. 1991 Jul 15;178(1):95-103. doi: 10.1016/0006-291x(91)91784-a.
A substance which is a potent inhibitor of Na+/K(+)-ATPase activity and competitively displaces [3H]ouabain binding to this enzyme was isolated from porcine blood cells. From its chemical and physiochemical properties, this activity was identified as hemin (chloroprotohemin IX). Hemin showed a dose dependent curve for Na+/K(+)-ATPase inhibitory activity similar to that of ouabain and displaced [3H]ouabain binding as potent as 1/100 of ouabain itself.
从猪血细胞中分离出一种物质，它是Na+/K(+)-ATP酶活性的强效抑制剂，能竞争性地取代[3H]哇巴因与该酶的结合。根据其化学和物理化学性质，该活性物质被鉴定为氯高铁血红素（氯原卟啉IX）。氯高铁血红素对Na+/K(+)-ATP酶的抑制活性呈现剂量依赖性曲线，类似于哇巴因，并且取代[3H]哇巴因结合的能力与哇巴因本身的1/100相当。
