由胚胎干细胞形成人类前列腺组织。

Formation of human prostate tissue from embryonic stem cells.

作者信息

Taylor Renea A, Cowin Prue A, Cunha Gerald R, Pera Martin, Trounson Alan O, Pedersen John, Risbridger Gail P

机构信息

Centre for Urological Research, Monash Institute of Medical Research, Monash University, 27-31 Wright Street, Clayton, Victoria, Australia 3168.

出版信息

Nat Methods. 2006 Mar;3(3):179-81. doi: 10.1038/nmeth855.

DOI:10.1038/nmeth855

PMID:16489334

Abstract

Rodent models and immortalized or genetically modified cell lines are frequently used-but have limited utility-for studying human prostate development and maturation. Using rodent mesenchyme to establish reciprocal mesenchymal-epithelial cell interactions with human embryonic stem cells (hESCs), we generated human prostate tissue expressing prostate-specific antigen (PSA) within 8-12 weeks. This human prostate model shows species-conserved signalling mechanisms that could extend to integumental, gastrointestinal and genital tissues.

摘要

啮齿动物模型以及永生化或基因改造的细胞系经常被用于研究人类前列腺的发育和成熟，但效用有限。利用啮齿动物间充质与人类胚胎干细胞（hESC）建立相互的间充质-上皮细胞相互作用，我们在8至12周内生成了表达前列腺特异性抗原（PSA）的人类前列腺组织。这种人类前列腺模型显示出物种保守的信号传导机制，可能延伸到皮肤、胃肠道和生殖组织。