Puthenveedu Manojkumar A, Bachert Collin, Puri Sapna, Lanni Frederick, Linstedt Adam D
Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, PA 15213, USA.
Nat Cell Biol. 2006 Mar;8(3):238-48. doi: 10.1038/ncb1366. Epub 2006 Feb 19.
The mammalian Golgi apparatus exists as stacks of cisternae that are laterally linked to form a continuous membrane ribbon, but neither the molecular requirements for, nor the purpose of, Golgi ribbon formation are known. Here, we demonstrate that ribbon formation is mediated by specific membrane-fusion events that occur during Golgi assembly, and require the Golgi proteins GM130 and GRASP65. Furthermore, these GM130 and GRASP65-dependent lateral cisternal-fusion reactions are necessary to achieve uniform distribution of enzymes in the Golgi ribbon. The membrane continuity created by ribbon formation facilitates optimal processing conditions in the biosynthetic pathway.
哺乳动物的高尔基体以一系列扁平囊堆叠的形式存在，这些扁平囊通过侧向连接形成连续的膜带，但高尔基体膜带形成的分子条件和目的均尚不清楚。在此，我们证明膜带形成是由高尔基体组装过程中发生的特定膜融合事件介导的，并且需要高尔基体蛋白GM130和GRASP65。此外，这些依赖GM130和GRASP65的侧向扁平囊融合反应对于在高尔基体膜带中实现酶的均匀分布是必需的。膜带形成所产生的膜连续性有利于生物合成途径中的最佳加工条件。
