Henderson T O, Kruski A W, Davis L G, Glonek T, Scanu A M
Biochemistry. 1975 May 6;14(9):1915-20. doi: 10.1021/bi00680a017.
A paramagnetic quenching reagent, Mn-2+/EDTA (1:2.2), was developed for the purpose of investigating the phospholipid phosphate groupings of human serum low and high density lipoproteins through the quenching effect of the reagent on the 31-P nuclear magnetic resonance signals from these complexes. Systems investigated included native low and high density serum liproteins (LDL, HDL2, and HDL3), egg phosphatidylcholine vesicles together with appropriate phosphodiester model systems, diethyl phosphate in aqueous buffer, and phosphatidylcholine and sphingomyelin both in anhydrous methanol. The results of these studies indicated that ca. 50 percent of the phospholipid-phosphorus signal of LDL is quenched upon titration as compared to an 80-85 percent figure observed for HDL2 and HDL3. In all cases the spectral effects were totally reversible upon removalof the paramagnetic ion by dialysis. The results of the titration studies indicated a similar but not an identical behavior between HDL2 and HDL3. The results are consistent with model structures of HDL and LDL particles derived from low angle X-ray diffraction.
开发了一种顺磁猝灭试剂Mn-2+/EDTA（1:2.2），目的是通过该试剂对来自这些复合物的31-P核磁共振信号的猝灭作用，研究人血清低密度和高密度脂蛋白的磷脂磷酸基团。所研究的体系包括天然的血清低密度和高密度脂蛋白（LDL、HDL2和HDL3）、卵磷脂囊泡以及合适的磷酸二酯模型体系、水缓冲液中的磷酸二乙酯，以及无水甲醇中的卵磷脂和鞘磷脂。这些研究结果表明，与HDL2和HDL3观察到的80-85%的数值相比，LDL的磷脂-磷信号在滴定后约50%被猝灭。在所有情况下，通过透析去除顺磁离子后，光谱效应完全可逆。滴定研究结果表明HDL2和HDL3之间有相似但不完全相同的行为。这些结果与源自低角度X射线衍射的HDL和LDL颗粒的模型结构一致。
