转化生长因子-β刺激人牙周膜和牙龈成纤维细胞产生白细胞介素-11。

Transforming growth factor-beta stimulates interleukin-11 production by human periodontal ligament and gingival fibroblasts.

作者信息

Yashiro R, Nagasawa T, Kiji M, Hormdee D, Kobayashi H, Koshy G, Nitta H, Ishikawa I

机构信息

Periodontology, Department of Hard Tissue Engineering, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan.

出版信息

J Clin Periodontol. 2006 Mar;33(3):165-71. doi: 10.1111/j.1600-051X.2006.00898.x.

DOI:10.1111/j.1600-051X.2006.00898.x

PMID:16489941

Abstract

BACKGROUND

Transforming growth factor (TGF)-beta is a potent multifunctional polypeptide, abundant in the bone matrix. Interleukin (IL)-11 is a pleiotropic cytokine with effects on multiple cell types. The present study was performed to evaluate the regulatory effects of TGF-beta on IL-11 production by human periodontal ligament cells (PDL) and human gingival fibroblasts (HGF).

MATERIAL AND METHODS

The expression of TGF-beta receptor in PDL and HGF were observed using flow cytometry. PDL and HGF were stimulated with TGF-beta with or without protein kinase C (PKC) inhibitors and activator. IL-11, bone morphogenetic protein-2 (BMP-2) and TGF-beta mRNA expression was quantified by real-time polymerase chain reaction (PCR). IL-11 production was measured using enzyme-linked immunosorbent assay.

RESULTS

PDL and HGF expressed both TGF-beta receptor I and TGF-beta receptor II on the cell surfaces. IL-11 mRNA expression and IL-11 production were augmented by TGF-beta in both PDL and HGF, with higher values in PDL. PKC inhibitors partially suppressed TGF-beta-induced IL-11 production in PDL and HGF, whereas activator enhanced it. TGF-beta mRNA and BMP-2 mRNA expression were up-regulated by TGF-beta in PDL.

CONCLUSION

These results suggest that PDL produce IL-11 in response to TGF-beta.

摘要

背景

转化生长因子（TGF）-β是一种强大的多功能多肽，在骨基质中含量丰富。白细胞介素（IL）-11是一种多效性细胞因子，对多种细胞类型有作用。本研究旨在评估TGF-β对人牙周膜细胞（PDL）和人牙龈成纤维细胞（HGF）产生IL-11的调节作用。

材料与方法

采用流式细胞术观察PDL和HGF中TGF-β受体的表达。用TGF-β刺激PDL和HGF，同时添加或不添加蛋白激酶C（PKC）抑制剂和激活剂。通过实时聚合酶链反应（PCR）定量IL-11、骨形态发生蛋白-2（BMP-2）和TGF-β mRNA的表达。使用酶联免疫吸附测定法测量IL-11的产生。

结果

PDL和HGF在细胞表面均表达TGF-β受体I和TGF-β受体II。TGF-β增强了PDL和HGF中IL-11 mRNA的表达和IL-11的产生，PDL中的值更高。PKC抑制剂部分抑制了TGF-β诱导的PDL和HGF中IL-11的产生，而激活剂则增强了这种产生。TGF-β上调了PDL中TGF-β mRNA和BMP-2 mRNA的表达。