Khoshnoodi Mohammad Ali, Motiei-Langroudi Rouzbeh, Tahsili-Fahadan Pouya, Yahyavi-Firouz-Abadi Noushin, Ghahremani Mohammad Hossein, Dehpour Ahmad Reza
Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Neurosci Lett. 2006 May 22;399(3):234-9. doi: 10.1016/j.neulet.2006.01.059. Epub 2006 Feb 21.
Agmatine recently has been suggested as a neurotransmitter, is able to interact with various effects of morphine like analgesia and dependence. In this study, the effects of agmatine on rewarding properties of morphine, and the possible involvement of nitric oxide (NO) system has been evaluated in an unbiased conditioned place preference (CPP) paradigm. Agmatine (1, 5 and 10mg/kg, i.p.) alone induced neither CPP nor conditioned place aversion (CPA). Morphine (0.01, 0.05, 0.1 and 0.5mg/kg, s.c.), while unable to show CPP or CPA, induced CPP in mice pretreated with agmatine. L-arginine (200mg/kg, i.p.), a NO precursor, significantly enhanced the effect of agmatine (5mg/kg) on morphine (0.5mg/kg)-induced place preference. NG-nitro-L-arginine methyl ester (L-NAME; 2.5mg/kg, i.p.), a non specific nitric oxide synthase (NOS) inhibitor, and aminoguanidine (50 and 100mg/kg, i.p.), a specific inducible NOS inhibitor, significantly reduced the effect of agmatine (5mg/kg) on morphine (0.5mg/kg)-induced place preference. These results suggest the possible involvement of inducible nitric oxide system in potentiating effects of agmatine on morphine-induced place preference.
胍丁胺最近被认为是一种神经递质，能够与吗啡的各种作用如镇痛和成瘾相互作用。在本研究中，采用无偏倚条件性位置偏爱（CPP）范式评估了胍丁胺对吗啡奖赏特性的影响以及一氧化氮（NO）系统可能的参与作用。单独给予胍丁胺（1、5和10mg/kg，腹腔注射）既不诱导CPP也不诱导条件性位置厌恶（CPA）。吗啡（0.01、0.05、0.1和0.5mg/kg，皮下注射）虽然不能表现出CPP或CPA，但能在预先用胍丁胺处理的小鼠中诱导CPP。NO前体L-精氨酸（200mg/kg，腹腔注射）显著增强了胍丁胺（5mg/kg）对吗啡（0.5mg/kg）诱导的位置偏爱的作用。非特异性一氧化氮合酶（NOS）抑制剂NG-硝基-L-精氨酸甲酯（L-NAME；2.5mg/kg，腹腔注射）和特异性诱导型NOS抑制剂氨基胍（50和100mg/kg，腹腔注射）显著降低了胍丁胺（5mg/kg）对吗啡（0.5mg/kg）诱导的位置偏爱的作用。这些结果提示诱导型一氧化氮系统可能参与了胍丁胺对吗啡诱导的位置偏爱的增强作用。
