Eichhorn M, Inada K, Lütjen-Drecoll E
Department of Anatomy, University of Erlangen-Nürnberg, FRG.
Curr Eye Res. 1991 Apr;10(4):277-86. doi: 10.3109/02713689108996333.
Ciliary body explants from 30 human eyes were maintained in organ culture up to 14 days. The age of the donors ranged from 45 to 85 years, the post mortem time from 4 to 22 hours. The ciliary epithelium as well as the underlying stroma were studied light- and electronmicroscopically before incubation and after 1, 3, 5, 7 and 14 days of culture. At the same time intervals, the localization of Na/K-ATPase and carbonic anhydrase (CA) were examined histochemically. If the cells were already damaged before incubation in medium (9 cases), they did not recover in culture. Best results were obtained after 3 to 5 days of culture with a survival rate of more than 90% after 3 days and more than 70% after 5 days, respectively. Both the nonpigmented (NPE) and the pigmented epithelium (PE) of the pars plicata in culture retained the morphological characteristics of epithelia involved in active secretion, namely elaborate infoldings of the cell membranes, numerous mitochondria in the cytoplasm and high activity of Na/K-ATPase and CA. In addition the adjacent capillaries were still fenestrated. After longer incubation times (7-14 days) the NPE and PE cells were filled with increasing amounts of lipid droplets and glycogen granules, indicating changes in metabolism.
来自30只人眼的睫状体外植体在器官培养中维持长达14天。供体年龄在45至85岁之间，死后时间为4至22小时。在培养前以及培养1、3、5、7和14天后，对睫状体上皮以及其下方的基质进行光镜和电镜研究。在相同的时间间隔，通过组织化学方法检查钠钾ATP酶和碳酸酐酶（CA）的定位。如果细胞在培养基中培养前已经受损（9例），它们在培养中不会恢复。培养3至5天后获得最佳结果，3天后存活率超过90%，5天后超过70%。培养中的睫状突非色素上皮（NPE）和色素上皮（PE）均保留了参与主动分泌的上皮细胞的形态学特征，即细胞膜有精细的内褶、细胞质中有大量线粒体以及钠钾ATP酶和CA的高活性。此外，相邻的毛细血管仍有窗孔。培养较长时间（7 - 14天）后，NPE和PE细胞内充满了越来越多的脂滴和糖原颗粒，表明代谢发生了变化。
