Lv Xuefei, Shao Jing, Song Maoyong, Zhou Qunfang, Jiang Guibin
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P. O. Box 2871, 100085, Beijing, PR China.
Comp Biochem Physiol C Toxicol Pharmacol. 2006 May;143(1):127-33. doi: 10.1016/j.cbpc.2006.01.002. Epub 2006 Feb 21.
Male Chinese loaches (Misgurnus anguillicaudatus) were exposed to 17beta-estradiol (E2) in the ambient water at nominal concentrations of 0.5, 1, 5 and 10 mug/L continuously for 8 weeks using a semi-static system. The gonadosomatic index (GSI), total plasma protein, total plasma calcium, magnesium and zinc, and plasma vitellogenin (Vtg) were determined as test endpoints. Our results indicate no significant changes in the GSI between exposed and control groups (P<0.05). All exposure concentrations of E2 dramatically induced the production of Vtg within 7 days. The vitellogenic response of male loach was time- and dose-dependent. At higher concentrations of E2 (5 and 10 microg/L), total plasma protein, total plasma calcium, and total plasma magnesium presented a significant time- and dose-dependent increase, and the three parameters were significantly correlated with plasma Vtg. We conclude that Chinese loaches are sensitive to estrogenic compounds and may be chosen as potential sentinel species in field and laboratory studies. In addition, total plasma protein, total plasma calcium, and total plasma magnesium can be used as indirect indicators to predict Vtg levels in estrogenized Chinese loach.
雄性中华泥鳅（Misgurnus anguillicaudatus）采用半静态系统，在环境水中连续8周暴露于名义浓度为0.5、1、5和10微克/升的17β-雌二醇（E2）中。测定性腺指数（GSI）、血浆总蛋白、血浆总钙、镁和锌以及血浆卵黄蛋白原（Vtg）作为测试终点。我们的结果表明，暴露组和对照组之间的GSI没有显著变化（P<0.05）。所有E2暴露浓度在7天内均显著诱导Vtg的产生。雄性泥鳅的卵黄生成反应具有时间和剂量依赖性。在较高浓度的E2（5和10微克/升）下，血浆总蛋白、血浆总钙和血浆总镁呈现出显著的时间和剂量依赖性增加，并且这三个参数与血浆Vtg显著相关。我们得出结论，中华泥鳅对雌激素化合物敏感，可作为野外和实验室研究中的潜在指示物种。此外，血浆总蛋白、血浆总钙和血浆总镁可作为间接指标来预测雌激素化中华泥鳅中的Vtg水平。
