Koizumi Noriko, Yamasaki Kenta, Kawasaki Satoshi, Sotozono Chie, Inatomi Tsutomu, Mochida Chikako, Kinoshita Shigeru
Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-0841, Japan.
Am J Ophthalmol. 2006 Mar;141(3):564-5. doi: 10.1016/j.ajo.2005.09.021.
To report cytomegalovirus (CMV) DNA in aqueous humor from a patient with unilateral corneal endotheliitis.
Case report.
A 51-year-old man presented with unilateral corneal endotheliitis with linear keratic precipitates and coin-shaped lesions. Tear and aqueous humor samples were subjected to polymerase chain reaction to look for DNA from herpes simplex virus (HSV), varicella zoster virus (VZV), and CMV.
Aqueous humor from the diseased eye contained DNA from CMV but not HSV or VZV. Its specificity was confirmed by Southern blot tests. Intravenous ganciclovir treatment resulted in the localization of his corneal edema and the reduction in keratic precipitates. There was severe destruction of corneal endothelial cells. CMV DNA was not detected in tears or control samples.
In this healthy man with corneal endotheliitis, we detected CMV DNA in aqueous humor from the affected eye, but not HSV or VZV. This suggests that CMV may cause corneal endotheliitis in patients without immunodeficiency.
报告一例单侧角膜内皮炎患者房水中的巨细胞病毒（CMV）DNA。
病例报告。
一名51岁男性患者出现单侧角膜内皮炎，伴有线状角膜后沉着物和钱币状病变。采集泪液和房水样本，进行聚合酶链反应，以检测单纯疱疹病毒（HSV）、水痘带状疱疹病毒（VZV）和CMV的DNA。
患眼房水中含有CMV的DNA，但未检测到HSV或VZV的DNA。通过Southern印迹试验证实了其特异性。静脉注射更昔洛韦治疗后，患者角膜水肿局限，角膜后沉着物减少。角膜内皮细胞有严重破坏。泪液或对照样本中未检测到CMV DNA。
在这名患有角膜内皮炎的健康男性患者中，我们在患眼房水中检测到CMV DNA，但未检测到HSV或VZV的DNA。这表明CMV可能在无免疫缺陷的患者中引起角膜内皮炎。
