Castro-Hermida J A, Pors I, Méndez-Hermida F, Ares-Mazás E, Chartier C
AFSSA site de NIORT, Laboratoire d'Etudes et de Recherches Caprines, 60 rue de Pied de Fond, B.P. 3081-79012 Niort Cedex, France.
Vet J. 2006 Mar;171(2):340-5. doi: 10.1016/j.tvjl.2004.11.003. Epub 2005 Jan 19.
Cryptosporidiosis is mainly a problem in neonatal ruminants. Not only do Cryptosporidium spp. spread ubiquitously in our environment, but the protozoa are highly resistant to harsh environmental conditions and disinfectants, and a control measure is urgently required. This study investigated the potential biocidal activity on Cryptosporidium parvum oocysts of two commercial disinfectants developed originally to be used in farms and food-processing industries. The products, containing formaldehyde and hydrogen peroxide respectively, both had some anticryptosporidial effects. The viability and infectivity of purified C. parvum oocysts exposed to both disinfectants at different concentrations and exposure times were evaluated by inclusion or exclusion of vital dye (propidium iodide), use of an excystation technique and infection of suckling mice. Viability assays showed a decrease in oocyst viability associated with an increase in exposure time for each of the concentrations used. The intensity of infection in neonatal mice was significantly lower (P<0.05) than in the control litters.
隐孢子虫病主要是新生反刍动物面临的一个问题。隐孢子虫属不仅在我们的环境中广泛传播，而且这种原生动物对恶劣的环境条件和消毒剂具有高度抗性，因此迫切需要一种控制措施。本研究调查了两种最初开发用于农场和食品加工业的商用消毒剂对微小隐孢子虫卵囊的潜在杀菌活性。这两种产品分别含有甲醛和过氧化氢，均具有一定的抗隐孢子虫作用。通过死活染料（碘化丙啶）的包入或排除、使用脱囊技术以及感染乳鼠，评估了纯化的微小隐孢子虫卵囊在不同浓度和暴露时间下接触这两种消毒剂后的活力和感染力。活力测定表明，在所使用的每种浓度下，卵囊活力均随着暴露时间的增加而降低。新生小鼠的感染强度显著低于（P<0.05）对照窝。
