Essendi Walter Miding'a, Muleke Charles, Miheso Manfred, Otachi Elick
Department of Biological Sciences, Egerton University, P.O. Box 536-20115, Egerton, Kenya.
Kenya Agricultural and Livestock Research Organization, Food Crops Research Centre Njoro, Njoro, Kenya.
J Parasit Dis. 2022 Mar;46(1):262-271. doi: 10.1007/s12639-021-01444-4. Epub 2021 Aug 28.
spp. cause cryptosporidiosis in humans through zoonotic and anthroponotic transmission. Previous studies illustrated the significance of domestic animals as reservoirs of this parasite. occurs in Njoro River; a main source of water to humans and animals. However, there is no information on the spp. and genotypes circulating in Njoro Sub County. A total of 2174 samples from humans, cattle, chickens, sheep and goats were assessed for the presence of spp. Thirty-three positive samples were subsequently successfully sequenced and compared to sequences in the GenBank repository using NCBI's (National Center for Biotechnology Information) online BLAST (Basic Local Alignment Search Tool) algorithmic program. Sequence alignment was done using the Clustal W program and phylogenetic analysis was executed in MEGA X (Molecular Evolutionary Genetics Analysis version X). The prevalence of cryptosporidiosis in Njoro Sub County is 6.99%. spp. present in the watershed showed great genetic diversity and nine species were recorded: , , . This is the first study to report the presence of in Kenya. Cattle is the major reservoir of zoonotic spp. while goats harbored the lowest number of species. Humans and domestic animals drink the contaminated water from Njoro River, humans are therefore, exposed to a high cryptosporidiosis risk.
隐孢子虫属通过人畜共患病和人传人传播途径导致人类患隐孢子虫病。先前的研究表明家畜作为这种寄生虫宿主的重要性。隐孢子虫出现在乔罗河，这是人类和动物的主要水源。然而，关于乔罗子县流行的隐孢子虫属种类和基因型尚无相关信息。共对来自人类、牛、鸡、绵羊和山羊的2174份样本进行了隐孢子虫属检测。随后对33份阳性样本成功进行了测序，并使用美国国立生物技术信息中心（NCBI）的在线BLAST（基本局部比对搜索工具）算法程序与GenBank数据库中的序列进行了比较。使用Clustal W程序进行序列比对，并在MEGA X（分子进化遗传学分析版本X）中进行系统发育分析。乔罗子县隐孢子虫病的患病率为6.99%。该流域存在的隐孢子虫属显示出高度的遗传多样性，记录到9种隐孢子虫：微小隐孢子虫、安氏隐孢子虫、贝氏隐孢子虫。这是肯尼亚首次报道隐孢子虫存在的研究。牛是动物源性隐孢子虫属的主要宿主，而山羊携带的种类最少。人类和家畜饮用来自乔罗河的受污染水，因此人类面临着很高的隐孢子虫病感染风险。
