Aizawa M, Coughlin R W, Charles M
Biochim Biophys Acta. 1975 Apr 7;385(2):362-70. doi: 10.1016/0304-4165(75)90365-7.
Reduced nicotinamide adenine dinucleotide (NADH) has been characterized electrochemically by solid electrode voltammetry and controlled potential electrolysis. Photometric and enzymatic assay showed that enzymatically active nicotinamide adenine dinucleotide (NAD-+) could be regenerated electrolytically from its reduced form without the use of so-called electron mediators. Complete regeneration of enzymatically active NAD can be expected in pyrophosphate buffers and phosphate buffers during the electrolysis. Advantages of electrochemical regeneration of coenzymes are discussed, especially with regard to immobilization of enzymes.
还原型烟酰胺腺嘌呤二核苷酸（NADH）已通过固体电极伏安法和控制电位电解法进行了电化学表征。光度法和酶法分析表明，无需使用所谓的电子介质，即可从其还原形式电解再生具有酶活性的烟酰胺腺嘌呤二核苷酸（NAD⁺）。在电解过程中，焦磷酸盐缓冲液和磷酸盐缓冲液中有望实现具有酶活性的NAD的完全再生。本文讨论了辅酶电化学再生的优点，特别是关于酶的固定化方面。
