Feinstein D L, Durand M, Milner R J
Department of Neuropharmacology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
Brain Res Mol Brain Res. 1991 May;10(2):97-105. doi: 10.1016/0169-328x(91)90099-j.
We have characterized cDNA clones of mRNAs encoding two distinct isoforms of myosin regulatory light chain expressed in rat brain. One clone, isolated from a cultured astrocyte cDNA library, is derived from a 1200-base mRNA that is expressed at high levels in cultured astrocytes, and at higher levels in the embryonic brain than in the adult brain. The nucleotide sequence of this cDNA is essentially identical to a previously reported cDNA encoding a smooth muscle isoform from rat aorta cells (Taubman et al., J. Cell Biol., 104 (1987) 1505-1515). The second clone hybridized to a 1300-base mRNA that is expressed abundantly in the adult brain and is the predominant species in cultured neuroblasts. Both mRNAs are expressed, to varying extents, in other muscle and nonmuscle tissues. The deduced amino acid sequences of the two isoforms differ in 4 residues out of 171. On the basis of the tissue distribution of their mRNAs and a comparison of identities among the known amino acid sequences of myosin regulatory light chains we suggest that both proteins should be considered as non-muscle isoforms. We conclude that there are at least two isoforms of the myosin regulatory light chain expressed in rat brain and that their expression is under both cell-specific and developmental regulation.
