Wang Lin, Cheng Jun, Li Ke, Hong Yuan
Center for Viral Hepatitis Research, Institute of Infectious Diseases, 302 Hospital of PLA, Beijing 100039, China.
Zhonghua Gan Zang Bing Za Zhi. 2006 Feb;14(2):81-5.
To clarify the expression and regulation mechanism of the new target gene (human hepatitis C virus binding protein 6, Hcbp6) interaction with the core protein of hepatitis C virus (HCV).
Referring to the prediction online of the promoter region, 3256 base pairs (bp) upstream and downstream of the translation start site were selected as 5 putative promoter sequences which were amplified from the hepatoblastoma cell line-HepG2 cell genomic DNA by polymerase chain reaction (PCR). The amplified products were cloned into a pCAT3 vector. The HepG2 and NIH3T3 cell lines were transfected by pCAT3-Hcbp6-promoters. The CAT activity was detected using an enzyme-linked immunoassay (ELISA) kit.
We found that 2 kinds of pCAT3-Hcbp6-promoters (1066 and 240) could direct the reporter gene expression. The expression of CAT was 3.1 times and 6.4 times higher than that of the pCAT3-basic control vector.
These results indicated that pCAT3-Hcbp6-promoters have promoter activity.
