[Identification and evaluation promoter sequence and the transcription activation of Hcbp6 interaction with core protein of hepatitis C virus].

OBJECTIVE

To clarify the expression and regulation mechanism of the new target gene (human hepatitis C virus binding protein 6, Hcbp6) interaction with the core protein of hepatitis C virus (HCV).

METHODS

Referring to the prediction online of the promoter region, 3256 base pairs (bp) upstream and downstream of the translation start site were selected as 5 putative promoter sequences which were amplified from the hepatoblastoma cell line-HepG2 cell genomic DNA by polymerase chain reaction (PCR). The amplified products were cloned into a pCAT3 vector. The HepG2 and NIH3T3 cell lines were transfected by pCAT3-Hcbp6-promoters. The CAT activity was detected using an enzyme-linked immunoassay (ELISA) kit.

RESULTS

We found that 2 kinds of pCAT3-Hcbp6-promoters (1066 and 240) could direct the reporter gene expression. The expression of CAT was 3.1 times and 6.4 times higher than that of the pCAT3-basic control vector.

CONCLUSION

These results indicated that pCAT3-Hcbp6-promoters have promoter activity.