Dou Jun, Wang Jing, Liu Peng-bo, Zhang Xing-jian
Department of Pathogenic Biology and Immunology, Southeast University School of Basic Medical Science, Nanjing 210009, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2006 Jan;40(1):38-41.
To analyze three kinds of genotype hepatitis C virus (HCV) core protein expressed in human hepatoma (Huh-7) cell line and to recognize HCV core proteins biological function and its pathogenic mechanism.
The Huh-7 cell expressed three kinds of core proteins were established respectively. Affymetrix human gene chip was used for identifying the gene expression dependently on Affymetrix's protocol. All genes changed by 3 or 1.5 folds between the transfected cells and a control cells were further analyzed, and annotated by using NetAffx analysis through Affymetrix website and were categorized based on their biological processes.
The HCV-1b core protein caused 16 genes up/down-regulated expression, of which the immune response genes of PF4V1 and SPP1 were up-regulated 3.4 or 4.4 folds respectively. The HCV-2a core protein had caused the immune response gene CXCL5 and apoptosis gene BTF a down-regulated expression of 3.4 and 3.1 folds respectively, but caused the apoptosis genes of HRK and LZTS1 an up-regulated expression of 3.2 and 3.4 folds respectively. As compared with HCV 1b or 2a core protein, HCV-4b core protein caused 111 genes expression changing and it had more obvious effects on gene expression. If we applied 1.5 fold change for a comparison gene expression, a few of the same gene expression profiles might be caused by these two core proteins.
The three kinds of HCV core protein should have its own expression character and be mainly shown in immune responses, signal transduction, apoptosis, etc. It should be helpful for our recognizing the HCV core protein biological function and its pathogenic mechanism.
分析在人肝癌（Huh-7）细胞系中表达的三种基因型丙型肝炎病毒（HCV）核心蛋白，以认识HCV核心蛋白的生物学功能及其致病机制。
分别建立表达三种核心蛋白的Huh-7细胞。按照Affymetrix公司的方案，使用Affymetrix人类基因芯片鉴定基因表达。进一步分析转染细胞与对照细胞之间表达变化3倍或1.5倍的所有基因，并通过Affymetrix网站使用NetAffx分析进行注释，并根据其生物学过程进行分类。
HCV-1b核心蛋白导致16个基因上调/下调表达，其中PF4V1和SPP1的免疫反应基因分别上调3.4倍或4.4倍。HCV-2a核心蛋白导致免疫反应基因CXCL5和凋亡基因BTF分别下调3.4倍和3.1倍，但导致凋亡基因HRK和LZTS1分别上调3.2倍和3.4倍。与HCV 1b或2a核心蛋白相比，HCV-4b核心蛋白导致111个基因表达变化，对基因表达的影响更明显。如果将基因表达变化1.5倍用于比较，这两种核心蛋白可能会导致一些相同的基因表达谱。
三种HCV核心蛋白应有其自身的表达特征，主要表现在免疫反应、信号转导、凋亡等方面。这有助于我们认识HCV核心蛋白的生物学功能及其致病机制。
