Lee Mon-Juan, Liu Chien-Hung, Wang Sin-Yuan, Huang Chung-Ter, Huang Haimei
Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan.
Biochem Biophys Res Commun. 2006 Apr 14;342(3):744-50. doi: 10.1016/j.bbrc.2006.01.173. Epub 2006 Feb 9.
The Soj and Spo0J proteins, together with one or more parS sequences, are crucial to chromosome segregation and the progression of cell cycle in many bacteria. In Helicobacter pylori, genes coding for Soj and a plasmid replication-partition-related protein containing a Spo0J or ParB conserved domain, together with two putative parS sites identified in this study, were found to be located within the origin-proximal 20-30% of the circular chromosome. Recombinant H. pylori Spo0J bound specifically to the two putative parS sequences and that of Bacillus subtilis. In addition, hydrolysis of ATP by H. pylori Soj was accelerated in the presence of parS and/or Spo0J. Protein-protein interactions, intracellular levels, and subcellular localization of Soj and Spo0J were analyzed through polyclonal antibodies directed against recombinant Soj and Spo0J. This study was the first implication of the existence of a functional parABS system in H. pylori.
Soj和Spo0J蛋白，连同一个或多个parS序列，对许多细菌的染色体分离和细胞周期进程至关重要。在幽门螺杆菌中，编码Soj的基因以及一个含有Spo0J或ParB保守结构域的质粒复制-分配相关蛋白，连同本研究中鉴定出的两个假定的parS位点，被发现位于环状染色体近端20%-30%的区域内。重组幽门螺杆菌Spo0J特异性结合两个假定的parS序列以及枯草芽孢杆菌的parS序列。此外，在存在parS和/或Spo0J的情况下，幽门螺杆菌Soj对ATP的水解加速。通过针对重组Soj和Spo0J的多克隆抗体分析了Soj和Spo0J的蛋白质-蛋白质相互作用、细胞内水平和亚细胞定位。本研究首次表明幽门螺杆菌中存在功能性parABS系统。
