Sapsford Kim E, Taitt Chris R, Fertig Stephanie, Moore Martin H, Lassman Michael E, Maragos Chris M, Shriver-Lake Lisa C
George Mason University, 10910 University Blvd, MS 4E3, Manassas, VA 20110, USA.
Biosens Bioelectron. 2006 Jun 15;21(12):2298-305. doi: 10.1016/j.bios.2005.10.021. Epub 2006 Feb 21.
Because of the potential health risks of aflatoxin B1 (AFB1), it is essential to monitor the level of this mycotoxin in a variety of foods. An indirect competitive immunoassay has been developed using the NRL array biosensor, offering rapid, sensitive detection and quantification of AFB1 in buffer, corn and nut products. AFB1-spiked foods were extracted with methanol and Cy5-anti-AFB1 added to the resulting sample. The extracted sample/antibody mix was passed over a waveguide surface patterned with immobilized AFB1. The resulting fluorescence signal decreased as the concentration of AFB1 in the sample increased. The limit of detection for AFB1 in buffer, 0.3 ng/ml, was found to increase to between 1.5 and 5.1 ng/g and 0.6 and 1.4 ng/g when measured in various corn and nut products, respectively.
由于黄曲霉毒素B1（AFB1）存在潜在的健康风险，监测各类食品中这种霉菌毒素的含量至关重要。利用NRL阵列生物传感器开发了一种间接竞争免疫测定法，可对缓冲液、玉米和坚果产品中的AFB1进行快速、灵敏的检测和定量。向添加了AFB1的食品中加入甲醇进行提取，并将Cy5标记的抗AFB1抗体添加到所得样品中。将提取的样品/抗体混合物通过固定有AFB1的波导表面。随着样品中AFB1浓度的增加，产生的荧光信号减弱。结果发现，缓冲液中AFB1的检测限为0.3 ng/ml，在各类玉米和坚果产品中检测时，该检测限分别增至1.5至5.1 ng/g和0.6至1.4 ng/g。
