长臂猿白血病病毒受体的小鼠同源物：基因定位及在啮齿动物中可能的受体功能。

The mouse homolog of the Gibbon ape leukemia virus receptor: genetic mapping and a possible receptor function in rodents.

作者信息

Adamson M C, Silver J, Kozak C A

机构信息

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Virology. 1991 Aug;183(2):778-81. doi: 10.1016/0042-6822(91)91010-e.

DOI:10.1016/0042-6822(91)91010-e

PMID:1649508

Abstract

The mouse homolog of the Gibbon ape leukemia virus (GALV) receptor (Glvr-1) was mapped to mouse Chromosome 2 (Chr 2) by Southern blot analysis of somatic cell hybrids and positioned on this chromosome using an interspecies genetic cross. Mouse Chr 2 also encodes a receptor (Rec-2) for the wild mouse virus M813. To investigate whether Glvr-1 and Rec-2 could be the same gene, we sought evidence for sequence homology between the env- genes of their respective viruses. Southern blot hybridization with GALV-derived env and pol-env probes failed to detect any homology between GALV and M813, but did show that all mouse species tested carry numerous copies of GALV-related sequences. We speculate that a functional receptor for GALV-related viruses was expressed during Mus evolution.

摘要

通过对体细胞杂种进行Southern印迹分析，将长臂猿白血病病毒（GALV）受体（Glvr-1）的小鼠同源物定位到小鼠2号染色体（Chr 2）上，并利用种间遗传杂交将其定位在该染色体上。小鼠Chr 2还编码野生小鼠病毒M813的受体（Rec-2）。为了研究Glvr-1和Rec-2是否可能是同一基因，我们寻找了它们各自病毒的env基因之间序列同源性的证据。用GALV衍生的env和pol-env探针进行Southern印迹杂交未能检测到GALV和M813之间的任何同源性，但确实表明所有测试的小鼠物种都携带大量GALV相关序列的拷贝。我们推测在小家鼠进化过程中表达了一种GALV相关病毒的功能性受体。