Choe Andrea, Phun Huy Q, Tieu David D, Hu Yan Hong, Carpenter Ellen M
Mental Retardation Research Center, Department of Psychiatry and Biobehavioral Science, UCLA School of Medicine, Los Angeles, CA 90095, USA.
Gene Expr Patterns. 2006 Oct;6(7):730-7. doi: 10.1016/j.modgep.2005.12.004. Epub 2006 Feb 21.
We have examined the expression of three paralogous Hox genes from E11.5 through E15.5 in the mouse spinal cord. These ages coincide with major phases of spinal cord neurogenesis, neuronal differentiation, cell migration, gliogenesis, and motor neuron cell death. The three genes, Hoxa10, Hoxc10, and Hoxd10, are all expressed in the lumbar spinal cord and have distinct expression patterns. Mutations in these three genes are known to affect motor neuron patterning. All three genes show lower levels of expression at the rostral limits of their domains, with selective regions of higher expression more caudally. Hoxa10 and Hoxd10 expression appears confined to postmitotic cell populations in the intermediate and ventral gray, while Hoxc10 is also expressed in proliferating cells in the dorsal ventricular zone. Hoxc10 and Hoxd10 expression is clearly excluded from the lateral motor columns at rostral lumbar levels but is present in this region more caudally. Double labeling demonstrates that Hoxc10 expression is correlated with ventrolateral LIM gene expression in the caudal part of the lumbar spinal cord.
我们研究了小鼠脊髓中三个同源Hox基因从胚胎第11.5天到第15.5天的表达情况。这些时期与脊髓神经发生、神经元分化、细胞迁移、神经胶质生成以及运动神经元细胞死亡的主要阶段相吻合。这三个基因，即Hoxa10、Hoxc10和Hoxd10，均在腰段脊髓中表达，且具有不同的表达模式。已知这三个基因的突变会影响运动神经元的模式形成。所有这三个基因在其表达域的头端边界表达水平较低，在更靠尾端的区域有选择性的高表达区域。Hoxa10和Hoxd10的表达似乎局限于中间和腹侧灰质中的有丝分裂后细胞群体，而Hoxc10也在背侧室管膜区的增殖细胞中表达。在腰段脊髓头端水平，Hoxc10和Hoxd10的表达明显排除在外侧运动柱之外，但在更靠尾端的该区域则有表达。双重标记显示，Hoxc10的表达与腰段脊髓尾部腹外侧LIM基因的表达相关。
