体外培养骨髓基质细胞的生物活性和成骨潜能的初步鉴定与表征
[Preliminary identification and characterization of in vitro cultured bone marrow stromal cells for their bioactivity and osteogenic potential].
作者信息
Tu Xiao-li, Liu Hong-wei, Li Cai-liang, Ren Xiao-hua
机构信息
Department of Stomatology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Jan;26(1):111-3.
OBJECTIVE
To induce the differentiation of bone marrow stromall cells (BMSCs) isolated from Beagles into osteoblasts in vitro and identify the osteogenic potential and bioactivity of the BMSCs.
METHODS
Primary cultured BMSCs isolated from Beagles were subcultured in mineralization medium to induce their differentiation into osteoblasts, whose morphological characteristics and proliferation status were observed by phase-contrast microscope. The osteogenic activity of the cells was evaluated with von Kossa staining of the mineralized nodules and determination of the alkaline phosphatase activity.
RESULT
BMSCs cultured in vitro showed obvious osteogenic capacity in DMEM. Von Kossa staining of the mineralized nodules and alkaline phosphatase detection of the passaged cells both yielded positive results.
CONCLUSION
BMSCs cultured in vitro contain osteogenic precursor cells, and the passaged cells possess osteogenic potential.
目的
诱导从比格犬分离的骨髓基质细胞（BMSCs）在体外分化为成骨细胞，并鉴定BMSCs的成骨潜能和生物活性。
方法
将从比格犬分离的原代培养BMSCs在矿化培养基中传代培养以诱导其分化为成骨细胞，通过相差显微镜观察其形态特征和增殖状态。用矿化结节的冯·科萨染色和碱性磷酸酶活性测定来评估细胞的成骨活性。
结果
体外培养的BMSCs在DMEM中显示出明显的成骨能力。矿化结节的冯·科萨染色和传代细胞的碱性磷酸酶检测均得到阳性结果。
结论
体外培养的BMSCs含有成骨前体细胞，传代细胞具有成骨潜能。
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