Samadpour M, Barbour M W, Nguyen T, Cao T M, Buck F, Depavia G A, Mazengia E, Yang P, Alfi D, Lopes M, Stopforth J D
Institute for Environmental Health Inc, Seattle, Washington 98155, USA.
J Food Prot. 2006 Feb;69(2):441-3. doi: 10.4315/0362-028x-69.2.441.
The objective of this study was to determine the prevalence of enterohemorrhagic Escherichia coli (EHEC), E. coli O157, Salmonella, and Listeria monocytogenes in retail food samples from Seattle, Wash. A total of 2,050 samples of ground beef (1,750 samples), mushrooms (100 samples), and sprouts (200 samples) were collected over a 12-month period and analyzed for the presence of these pathogens. PCR assays, followed by culture confirmation were used to determine the presence or absence of each organism. Of the 1,750 ground beef samples analyzed, 61 (3.5%) were positive for EHEC, and 20 (1.1%) of these were positive for E. coli O157. Salmonella was present in 67 (3.8%) of the 1,750 ground beef samples. Of 512 ground beef samples analyzed, 18 (3.5%) were positive for L. monocytogenes. EHEC was found in 12 (6.0%) of the 200 sprout samples, and 3 (1.5%) of these yielded E. coli O157. Of the 200 total sprout samples, 14 (7.0%) were positive for Salmonella and none were positive for L. monocytogenes. Among the 100 mushroom samples, 4 (4.0%) were positive for EHEC but none of these 4 samples were positive for E. coli O157. Salmonella was detected in 5 (5.0%) of the mushroom samples, and L. monocytogenes was found in 1 (1.0%) of the samples.
本研究的目的是确定华盛顿州西雅图市零售食品样本中肠出血性大肠杆菌（EHEC）、大肠杆菌O157、沙门氏菌和单核细胞增生李斯特菌的流行情况。在12个月的时间里，共采集了2050份碎牛肉样本（1750份）、蘑菇样本（100份）和豆芽样本（200份），并对这些病原体的存在情况进行了分析。采用聚合酶链反应（PCR）检测，随后进行培养确认，以确定每种微生物的存在与否。在分析的1750份碎牛肉样本中，61份（3.5%）EHEC呈阳性，其中20份（1.1%）大肠杆菌O157呈阳性。在1750份碎牛肉样本中，67份（3.8%）存在沙门氏菌。在分析的512份碎牛肉样本中，18份（3.5%）单核细胞增生李斯特菌呈阳性。在200份豆芽样本中，12份（6.0%）检测到EHEC，其中3份（1.5%）产生大肠杆菌O157。在总共200份豆芽样本中，14份（7.0%）沙门氏菌呈阳性，单核细胞增生李斯特菌均为阴性。在100份蘑菇样本中，4份（4.0%）EHEC呈阳性，但这4份样本中没有一份大肠杆菌O157呈阳性。在5份（5.0%）蘑菇样本中检测到沙门氏菌，在1份（1.0%）样本中发现单核细胞增生李斯特菌。
