[一株以ectoine为主要相容性溶质积累的中度嗜盐细菌的分离与特性]

[Isolation and characteristic of a moderately halophilic bacterium accumulated ectoine as main compatible solute].

作者信息

He Jian, Wang Ting, Sun Ji-Quan, Gu Li-Feng, Li Shun-Peng

机构信息

Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Wei Sheng Wu Xue Bao. 2005 Dec;45(6):900-4.

PMID:16496700

Abstract

A moderately halophilic bacterium(designated strain I15) was isolated from lawn soil. Based on the analysis of 16S rDNA (GenBank accession number DQ010162), morphology, physiological and biochemical characteristics, strain I15 was identified as Virgibacillus marismortuii. This strain was capable of growing under 0% approximately 25% NaCl, and exhibited an optimum NaCl concentration of 10% and an optimum temperature of 30 degrees C and an optimum pH of 7.5 - 8.0 for its growth, respectively. Under hyperosmotic stress, strain 115 accumulated ectoine as the main compatible solute. Under 15% NaCl conditions the intracellar ectoine can reach to 1.608 mmol/(g x cdw), accounted for 89.6% of the total compatible solutes. The biosynthesis of ectoine was under the control of osmotic, and the accumulated ectoine synthesized intraceilularly can released under hypoosmotic shocks and resynthesis under hyperosmotic shock rapidly.

摘要

从草地土壤中分离出一株中度嗜盐菌（命名为菌株I15）。基于16S rDNA分析（GenBank登录号DQ010162）、形态学、生理生化特征，菌株I15被鉴定为死海芽孢杆菌。该菌株能够在约0%至25%的NaCl浓度下生长，其生长的最适NaCl浓度为10%，最适温度为30℃，最适pH为7.5 - 8.0。在高渗胁迫下，菌株I15积累ectoine作为主要的相容性溶质。在15% NaCl条件下，细胞内ectoine可达1.608 mmol/(g x cdw)，占总相容性溶质的89.6%。ectoine的生物合成受渗透压控制，细胞内积累的ectoine在低渗冲击下可释放，并在高渗冲击下迅速重新合成。