Yang Li, Zhao Yu-Hua, Zhang Bing-Xin, Zhang Xin
College of Life Science, Zhejiang University, Hangzhou 310029, China.
Wei Sheng Wu Xue Bao. 2005 Dec;45(6):905-9.
A strain DSP3 capable of utilizing chlorpyrifos as the sole carbon and energy sources was isolated. Based on the results of phenotypic features, phylogenetic of 16S rDNA sequence, DNA (G + C) mol% and DNA homology between strain DSP3 and reference strains, the strain DSP3 is identified as Alcaligenes faecalis. The degradation rate of chlorpyrifos was at 98.6% (100 mg/L) in liquid culture medium within 18 days and nearly 100% (100 mg/kg) in soil within 20 days respectively. An addition of strain DSP3 (10(8) cells/g) to soil resulted in a higher degradation rate than noninoculated soils. The different degrading rate of chlorpyrifos in four types of treated soils suggests that the dissipation is mediated by the activity of the soil microorganisms.
分离出了一株能够利用毒死蜱作为唯一碳源和能源的DSP3菌株。基于表型特征、16S rDNA序列系统发育、DNA（G + C）mol%以及DSP3菌株与参考菌株之间的DNA同源性结果，将DSP3菌株鉴定为粪产碱菌。在液体培养基中，18天内毒死蜱的降解率为98.6%（100 mg/L），在土壤中20天内降解率接近100%（100 mg/kg）。向土壤中添加DSP3菌株（10⁸个细胞/g）导致降解率高于未接种土壤。四种处理土壤中毒死蜱的不同降解率表明，其消散是由土壤微生物的活性介导的。
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