Pope C E, Gomez M C, Dresser B L
Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA.
Theriogenology. 2006 Oct;66(6-7):1518-24. doi: 10.1016/j.theriogenology.2006.01.026. Epub 2006 Feb 23.
Over a 5-year interval, multiple laparoscopic oocyte retrievals were done in fishing cats (Prionailurus viverrinus), caracals (Caracal caracal) and domestic cats after ovarian stimulation with gonadotropins. From 21 retrievals in five fishing cats, 579 preovulatory oocytes (mean = 27.6) were recovered and 348 embryos were produced in vitro (mean = 16.6). A total of 452 preovulatory oocytes (mean = 25.1) were recovered from 18 of 24 retrievals in six caracals and 297 (mean = 16.6) embryos were produced. An additional 16 caracal embryos (19%) were produced after in vitro maturation of 83 oocytes, 59 of which came from six retrievals producing only immature oocytes. The presence of corpora lutea at oocyte retrieval occurred in each species (1) at a similar frequency (33%) and (2) more frequently during January through May (11 of 15 retrievals) than during the latter half of the year (4 of 30 retrievals). Of the 12 embryo transfer procedures done in fishing cats, one pregnancy (8%) was obtained and one live kitten born after the auto-transfer of 10 Day-6 embryos. In caracals, a total of 46 Day-4 or Day-5 embryos were auto-transferred to six recipients, one of which delivered two live kittens. Then, 109 caracal embryos were cryopreserved before thawing and transferring to nine recipients (mean = 12.1) on Days 5 or 6. From three pregnancies established (33%), a total of three kittens were born. Two to six gonadotropin treatments/oocyte retrievals were done in domestic cats during 1999 through 2003; an average of 24.9, 23.5, 22.0, 23.1, 23.5 and 40.9 oocytes (P > 0.05) were recovered at the first through the sixth treatment cycles from 138, 138, 97, 49, 22, and seven retrievals, respectively.
在5年的时间里,对渔猫(豹猫属豹猫)、狞猫(狞猫属狞猫)和家猫使用促性腺激素进行卵巢刺激后,进行了多次腹腔镜取卵。在5只渔猫的21次取卵中,回收了579个排卵前卵母细胞(平均=27.6),并在体外产生了348个胚胎(平均=16.6)。在6只狞猫的24次取卵中的18次取卵中,共回收了452个排卵前卵母细胞(平均=25.1),并产生了297个(平均=16.6)胚胎。在83个卵母细胞体外成熟后,又产生了16个狞猫胚胎(19%),其中59个来自6次仅产生未成熟卵母细胞的取卵。在每个物种的取卵时黄体的存在情况如下:(1)频率相似(33%);(2)1月至5月期间出现的频率更高(15次取卵中的11次),高于下半年(30次取卵中的4次)。在对渔猫进行的12次胚胎移植手术中,自体移植10个第6天的胚胎后获得了1次妊娠(8%),并产下1只活小猫。在狞猫中,总共46个第4天或第5天的胚胎被自体移植到6只受体动物体内,其中1只产下了2只活小猫。然后,109个狞猫胚胎在解冻前被冷冻保存,并在第5天或第6天移植到9只受体动物体内(平均=12.1)。在已确定的3次妊娠(33%)中,总共产下了3只小猫。1999年至2003年期间,在家猫中进行了2至6次促性腺激素治疗/取卵;在第1至第6个治疗周期中,分别从138次、138次、97次、49次、22次和7次取卵中回收了平均24.9、23.5、22.0、23.1、23.5和40.9个卵母细胞(P>0.05)。