Couraud P O, Durieu-Trautmann O, Nguyen D L, Marin P, Glibert F, Strosberg A D
Laboratoire d'Immuno-Pharmacologie moléculaire, CNRS et Université Paris VII, Institut Cochin de Génétique Moléculaire (ICGM), France.
Eur J Pharmacol. 1991 Mar 25;206(3):191-8. doi: 10.1016/s0922-4106(05)80018-5.
Rat astrocytoma C6 cells have been recently identified as target cells for ET-1, which stimulates inositol lipid turnover in these cells. It is shown here that binding of ET-1 to high-affinity receptors on C6 cells leads to 40-45% inhibition of isoproterenol-induced intracellular cyclic AMP accumulation, as well as to stimulation of inositol lipid turnover, both effects characterized by an absolute requirement of extracellular calcium. Moreover, ET-1, which has been generally reported to have a mitogenic effect on a variety of target cells including primary rat astrocytes, is shown here to stimulate or, alternatively, inhibit DNA synthesis in C6 cells, depending on the subclone considered.
大鼠星形细胞瘤C6细胞最近被确定为内皮素-1（ET-1）的靶细胞，ET-1可刺激这些细胞中的肌醇脂质周转。本文表明，ET-1与C6细胞上的高亲和力受体结合会导致异丙肾上腺素诱导的细胞内环磷酸腺苷（cAMP）积累受到40%-45%的抑制，同时也会刺激肌醇脂质周转，这两种效应的特征是绝对需要细胞外钙。此外，一般报道ET-1对包括原代大鼠星形胶质细胞在内的多种靶细胞具有促有丝分裂作用，但本文表明，根据所考虑的亚克隆不同，ET-1在C6细胞中可刺激或抑制DNA合成。
