Li Long, Ma Guijuan, Gong Bolin
Key Laboratory of Energy & Chemical Engineering, Ningxia University, Yinchuan 750021, China.
Se Pu. 2005 Nov;23(6):567-72.
Monodisperse polystyrene seed particles were prepared by dispersion polymerization of styrene in ethanol and water under a nitrogen atmosphere using benzoyl peroxide as an initiator. Then the monodisperse, porous poly (chloromethylstyrene-co-divinylbenzene) beads in the range of 6 - 15 microm were prepared by one-step swelling and polymerization method. Based on this media, a new hydrophilic weak cation exchange stationary phase (WCX) for high performance liquid chromatography was synthesized by a new chemical modification method. The stationary phase was evaluated on the behavior of ion exchange, separability, and hydrophilicity. Effects of the nature of salts and pH value on the separation and retention of proteins of the stationary phase were investigated in details. It was found that the WCX resin follows ion exchange chromatographic retention mechanism. It was also used for the rapid separation and purification of lysozyme from egg white in only one step. The purity was found more than 96% and specific bioactivity of the purified lysozyme was as high as 71 184 U/mg.
在氮气氛围下，以过氧化苯甲酰为引发剂，通过苯乙烯在乙醇和水中的分散聚合制备了单分散聚苯乙烯种子颗粒。然后采用一步溶胀聚合法制备了粒径范围为6 - 15微米的单分散多孔聚（氯甲基苯乙烯 - 二乙烯基苯）珠粒。基于此介质，通过一种新的化学改性方法合成了一种用于高效液相色谱的新型亲水性弱阳离子交换固定相（WCX）。对该固定相的离子交换行为、分离能力和亲水性进行了评估。详细研究了盐的性质和pH值对固定相分离和保留蛋白质的影响。发现WCX树脂遵循离子交换色谱保留机制。它还用于仅一步快速从蛋清中分离和纯化溶菌酶。发现纯度超过96%，纯化溶菌酶的比生物活性高达71184 U/mg。
