Pesek Joseph J, Matyska Maria T
Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
Se Pu. 2005 Nov;23(6):595-608.
Silica hydride is a recent development in chromatographic support materials for high performance liquid chromatography (HPLC) where hydride groups replace 95% of the silanols on the surface. This conversion changes many of the fundamental properties of the material as well as the bonded stationary phases that are the result of further chemical modification of the hydride surface. Some unique chromatographic properties of hydride-based phases are described as well as some general application areas where these bonded materials may be used in preference to or have advantages not available from typical stationary phases. The fabrication, properties and applications of etched chemically modified capillaries for electrophoretic analysis are also reviewed. It is shown that the etching process creates a surface that is fundamentally different than a bare fused silica capillary. The new surface matrix produces unique electroosmotic flow properties and is more compatible with basic and biological compounds. After chemical modification of the surface, the bonded organic moiety (stationary phase) contributes to the control of migration of solutes in the capillary. Both electrophoretic and chromatographic processes take place in the etched chemically modified capillaries leading to a variety of experimental variables that can be used to optimize separations. A number of examples of separations on these capillaries are described.
氢化硅胶是用于高效液相色谱（HPLC）的色谱支持材料方面的一项最新进展，其中氢化物基团取代了表面95%的硅醇。这种转变改变了材料的许多基本特性以及键合固定相，而键合固定相是氢化物表面进一步化学修饰的结果。本文描述了基于氢化物的固定相的一些独特色谱特性，以及一些一般应用领域，在这些领域中，这些键合材料可能比典型固定相更受青睐或具有典型固定相所没有的优势。本文还综述了用于电泳分析的蚀刻化学修饰毛细管的制备、特性及应用。结果表明，蚀刻过程产生的表面与裸露的熔融石英毛细管表面有根本不同。新的表面基质产生独特的电渗流特性，并且与碱性和生物化合物更兼容。表面化学修饰后，键合的有机部分（固定相）有助于控制溶质在毛细管中的迁移。电泳和色谱过程都在蚀刻化学修饰的毛细管中进行，从而产生了各种可用于优化分离的实验变量。本文描述了在这些毛细管上进行分离的多个实例。
