甲状旁腺激素可增强负鼠肾细胞对庆大霉素的摄取，但对LLC - PK1细胞则无此作用。

Parathyroid hormone enhances gentamicin uptake by opossum kidney cells but not by LLC-PK1 cells.

作者信息

Nakahama H, Kakihara M, Fukuhara Y, Ueda N, Orita Y, Kamada T

机构信息

First Department of Medicine, Osaka University Medical School, Japan.

出版信息

Nephron. 1991;58(1):85-9. doi: 10.1159/000186383.

DOI:10.1159/000186383

PMID:1649976

Abstract

Parathyroidectomy is proposed to be protective against gentamicin nephrotoxicity in rats. In the present study, we evaluated the effects of bovine PTH(1-34) on gentamicin accumulation in cultured kidney epithelial cell lines, opossum kidney (OK) cells which possess PTH receptors and LLC-PK1 cells which are devoid of PTH receptors. Ten days after seeding, the culture medium was exchanged for medium containing 1 mM gentamicin and bovine PTH. The cell gentamicin concentration was measured by a substrate-labeled fluorescence immunoassay (TDA gentamicin kit). Gentamicin uptake was accelerated by bovine PTH in OK cells but not in LLC-PK1 cells. The enhancing effect of bovine PTH seems to be mediated by a cAMP-dependent process. The results suggest that PTH accelerates gentamicin accumulation in renal tissues and potentiates gentamicin nephrotoxicity.

摘要

甲状旁腺切除术被认为对大鼠庆大霉素肾毒性具有保护作用。在本研究中，我们评估了牛甲状旁腺激素（1-34）对培养的肾上皮细胞系（具有甲状旁腺激素受体的负鼠肾细胞（OK细胞）和缺乏甲状旁腺激素受体的LLC-PK1细胞）中庆大霉素蓄积的影响。接种10天后，将培养基换成含有1 mM庆大霉素和牛甲状旁腺激素的培养基。通过底物标记荧光免疫测定法（TDA庆大霉素试剂盒）测量细胞内庆大霉素浓度。牛甲状旁腺激素可加速OK细胞对庆大霉素的摄取，但对LLC-PK1细胞无此作用。牛甲状旁腺激素的增强作用似乎是由环磷酸腺苷（cAMP）依赖性过程介导的。结果表明，甲状旁腺激素可加速庆大霉素在肾组织中的蓄积并增强庆大霉素的肾毒性。